Protein Info for OHPLBJKB_03770 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: 5-keto-D-gluconate 5-reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 254 PF00106: adh_short" amino acids 10 to 203 (194 residues), 220.1 bits, see alignment E=3e-69 PF08659: KR" amino acids 12 to 163 (152 residues), 46.3 bits, see alignment E=7.3e-16 PF13561: adh_short_C2" amino acids 16 to 250 (235 residues), 199 bits, see alignment E=1.4e-62

Best Hits

Swiss-Prot: 100% identical to IDNO_ECOLI: 5-keto-D-gluconate 5-reductase (idnO) from Escherichia coli (strain K12)

KEGG orthology group: K00046, gluconate 5-dehydrogenase [EC: 1.1.1.69] (inferred from 100% identity to eco:b4266)

MetaCyc: 100% identical to 5-keto-D-gluconate 5-reductase (Escherichia coli K-12 substr. MG1655)
Gluconate 5-dehydrogenase. [EC: 1.1.1.69]; RXN0-7101 [EC: 1.1.1.69]

Predicted SEED Role

"5-keto-D-gluconate 5-reductase (EC 1.1.1.69)" in subsystem D-gluconate and ketogluconates metabolism (EC 1.1.1.69)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (254 amino acids)

>OHPLBJKB_03770 5-keto-D-gluconate 5-reductase (Escherichia coli HS(pFamp)R (ATCC 700891))
MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAERAELAVEKLHQEGIQA
VAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPFTEFPEQEWNDVIAVNQTA
VFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAASKGAVKMLTRGMCVELARHN
IQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARWGDPQELIGAAVFLSSKASDFVN
GHLLFVDGGMLVAV