Protein Info for OHPLBJKB_02914 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: putative ATP-dependent helicase DinG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 761 transmembrane" amino acids 37 to 59 (23 residues), see Phobius details PF00270: DEAD" amino acids 76 to 147 (72 residues), 27.4 bits, see alignment E=5.2e-10 PF06733: DEAD_2" amino acids 243 to 309 (67 residues), 31.1 bits, see alignment E=3.7e-11 PF13307: Helicase_C_2" amino acids 576 to 733 (158 residues), 122.9 bits, see alignment E=3e-39

Best Hits

Swiss-Prot: 100% identical to DING_ECOLI: ATP-dependent DNA helicase DinG (dinG) from Escherichia coli (strain K12)

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 100% identity to eco:b0799)

MetaCyc: 100% identical to ATP-dependent DNA helicase DinG (Escherichia coli K-12 substr. MG1655)
RXN0-4261 [EC: 5.6.2.3]

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (761 amino acids)

>OHPLBJKB_02914 putative ATP-dependent helicase DinG (Escherichia coli HS(pFamp)R (ATCC 700891))
MLERALNRIMLVVIASRFPETEKPLPLKTEKCHNIGCLYSISGFLMALTAALKAQIAAWY
KALQEQIPDFIPRAPQRQMIADVAKTLAGEEGRHLAIEAPTGVGKTLSYLIPGIAIAREE
QKTLVVSTANVALQDQIYSKDLPLLKKIIPDLKFTAAFGRGRYVCPRNLTALASTVPTQQ
DLLAFLDDELTPNNQEEQKRCAKLKGDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCL
NRNCYYYRECPFFVARREIQEAEVVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPD
VARDALEMSAEITAPWYRLQLDLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELI
ASLNNILNLYMPAGQEAEHRFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTG
SHDIVRLHRLILQMNRALGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHC
VGIRVSDQLERLLWRSIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHC
EQGKIVIPRMRVEPSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVT
DLRLMLLVQGDQPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAF
PPIDSPVVITEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLL
TKNYGKRLLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR