Protein Info for OHPLBJKB_02790 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Macrolide export protein MacA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 34 to 356 (323 residues), 192.5 bits, see alignment E=4.5e-61 PF16576: HlyD_D23" amino acids 41 to 288 (248 residues), 59.7 bits, see alignment E=3.8e-20 PF13533: Biotin_lipoyl_2" amino acids 56 to 102 (47 residues), 33.3 bits, see alignment 4.9e-12 PF13437: HlyD_3" amino acids 180 to 278 (99 residues), 53.5 bits, see alignment E=5.4e-18

Best Hits

Swiss-Prot: 100% identical to MACA_ECO57: Macrolide export protein MacA (macA) from Escherichia coli O157:H7

KEGG orthology group: K13888, macrolide-specific efflux protein MacA (inferred from 99% identity to eco:b0878)

MetaCyc: 99% identical to ABC-type tripartite efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
7.6.2.-; 7.6.2.-; 7.6.2.-

Predicted SEED Role

"Macrolide-specific efflux protein MacA" in subsystem Multidrug Resistance Efflux Pumps

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (365 amino acids)

>OHPLBJKB_02790 Macrolide export protein MacA (Escherichia coli HS(pFamp)R (ATCC 700891))
MKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPGDLQQSVLATGKLDALRKVDVGA
QVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIKEVEATLMELRAQRQQAEAELKLAR
VTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQIGTIDAQIKRNQASLDTAKTNLDYTRI
VAPMAGEVTQITTLQGQTVIAAQQAPNILTLADMSTMLVKAQVSEADVIHLKPGQKAWFT
VLGDPLTRYEGQIKDVLPTPEKVNDAIFYYARFEVPNPNGLLRLDMTAQVHIQLTDVKNV
LTIPLSALGDPVGDNRYKVKLLRNGETREREVTIGARNDTDVEIVKGLEAGDEVVIGEAK
PGAAQ