Protein Info for OHPLBJKB_02742 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 615 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF20142: Scaffold" amino acids 93 to 224 (132 residues), 65.2 bits, see alignment E=1.6e-21 PF01471: PG_binding_1" amino acids 313 to 348 (36 residues), 30.9 bits, see alignment 3.7e-11 PF03734: YkuD" amino acids 376 to 542 (167 residues), 90.5 bits, see alignment E=2.5e-29

Best Hits

Swiss-Prot: 100% identical to YCBB_ECOLI: Probable L,D-transpeptidase YcbB (ycbB) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b0925)

MetaCyc: 100% identical to L,D-transpeptidase LdtD (Escherichia coli K-12 substr. MG1655)
Muramoyltetrapeptide carboxypeptidase. [EC: 3.4.17.13]; RXN-16660 [EC: 3.4.17.13]; RXN-16665 [EC: 3.4.17.13]

Predicted SEED Role

"L,D-transpeptidase YcbB"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.4.17.13

Use Curated BLAST to search for 3.4.17.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (615 amino acids)

>OHPLBJKB_02742 hypothetical protein (Escherichia coli HS(pFamp)R (ATCC 700891))
MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAVSEQGEALPQAQATAIM
AGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARDMQPMWENRDAVKAFQQQL
AEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGYLHFIANIPVKGTRWLYSSKP
YALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYAAMHESLLALLCDTKPWPQLTGK
ATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGDDTPTDAVVSPSAVTVETAETKPMD
KQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGLGADGAIGPATRDWLNVTPAQRAGVLA
LNIQRLRLLPTELSTGIMVNIPAYSLVYYQNGNQVLDSRVIVGRPDRKTPMMSSALNNVV
VNPPWNVPPTLARKDILPKVRNDPGYLESHGYTVMRGWNSREAIDPWQVDWSTITASNLP
FRFQQAPGPRNSLGRYKFNMPSSEAIYLHDTPNHNLFKRDTRALSSGCVRVNKASDLANM
LLQDAGWNDKRISDALKQGDTRYVNIRQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPA
RSSSQIVSKAEQLIR