Protein Info for OHPLBJKB_02731 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Alkanesulfonate monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 381 TIGR03565: alkanesulfonate monooxygenase, FMNH(2)-dependent" amino acids 4 to 349 (346 residues), 630.5 bits, see alignment E=3.3e-194 PF00296: Bac_luciferase" amino acids 5 to 325 (321 residues), 280.2 bits, see alignment E=1.3e-87

Best Hits

Swiss-Prot: 100% identical to SSUD_ECOBW: Alkanesulfonate monooxygenase (ssuD) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K04091, alkanesulfonate monooxygenase [EC: 1.14.14.5] (inferred from 100% identity to eco:b0935)

MetaCyc: 100% identical to FMNH2-dependent alkanesulfonate monooxygenase (Escherichia coli K-12 substr. MG1655)
Alkanesulfonate monooxygenase. [EC: 1.14.14.5]; 1.14.14.5 [EC: 1.14.14.5]

Predicted SEED Role

"Alkanesulfonate monooxygenase (EC 1.14.14.5)" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization (EC 1.14.14.5)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.14.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (381 amino acids)

>OHPLBJKB_02731 Alkanesulfonate monooxygenase (Escherichia coli HS(pFamp)R (ATCC 700891))
MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGYTGVLIPTGRSCEDAWL
VAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGRALFNLVTGSDPQELAGDG
VFLDHSERYEASAEFTQVWRRLLQRETVDFNGKHIHVRGAKLLFPAIQQPYPPLYFGGSS
DVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAHGRKIRFGIRLHVIVRETNDEAW
QAAERLISHLDDETIAKAQAAFARTDSVGQQRMAALHNGKRDNLEISPNLWAGVGLVRGG
AGTALVGDGPTVAARINEYAALGIDSFVLSGYPHLEEAYRVGELLFPLLDVAIPEIPQPQ
PLNPQGEAVANDFIPRKVAQS