Protein Info for OHPLBJKB_02645 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Poly-beta-1,6-N-acetyl-D-glucosamine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 441 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 32 to 54 (23 residues), see Phobius details amino acids 322 to 345 (24 residues), see Phobius details amino acids 360 to 383 (24 residues), see Phobius details amino acids 395 to 418 (24 residues), see Phobius details TIGR03937: poly-beta-1,6 N-acetyl-D-glucosamine synthase" amino acids 30 to 437 (408 residues), 647.8 bits, see alignment E=3.6e-199 PF13641: Glyco_tranf_2_3" amino acids 75 to 298 (224 residues), 124.5 bits, see alignment E=1.9e-39 PF10111: Glyco_tranf_2_2" amino acids 78 to 176 (99 residues), 39.1 bits, see alignment E=1.9e-13 PF00535: Glycos_transf_2" amino acids 78 to 245 (168 residues), 135.8 bits, see alignment E=4.5e-43 PF03142: Chitin_synth_2" amino acids 113 to 257 (145 residues), 28.8 bits, see alignment E=1.6e-10 PF13506: Glyco_transf_21" amino acids 142 to 297 (156 residues), 50.9 bits, see alignment E=4e-17 PF13632: Glyco_trans_2_3" amino acids 158 to 372 (215 residues), 88.1 bits, see alignment E=2.2e-28

Best Hits

Swiss-Prot: 100% identical to PGAC_ECOLI: Poly-beta-1,6-N-acetyl-D-glucosamine synthase (pgaC) from Escherichia coli (strain K12)

KEGG orthology group: K11936, biofilm PGA synthesis N-glycosyltransferase PgaC [EC: 2.4.-.-] (inferred from 100% identity to eco:b1022)

MetaCyc: 100% identical to poly-N-acetyl-D-glucosamine synthase subunit PgaC (Escherichia coli K-12 substr. MG1655)
2.4.1.M63 [EC: 2.4.1.M63]; TRANS-RXN0-549 [EC: 2.4.1.M63]

Predicted SEED Role

"Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-)" (EC 2.4.-.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.-.-

Use Curated BLAST to search for 2.4.-.- or 2.4.1.M63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (441 amino acids)

>OHPLBJKB_02645 Poly-beta-1,6-N-acetyl-D-glucosamine synthase (Escherichia coli HS(pFamp)R (ATCC 700891))
MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHW
PWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRA
ILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPM
LYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRS
ALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGG
AEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIA
ATHTAGILLCTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRV
MLMPKKQRARWVSPDRGILRG