Protein Info for OHPLBJKB_02447 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Thymidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00265: TK
  • amino acids 2 to 186 (185 residues), 221.3 bits, E=4.1e-70

Best Hits

Swiss-Prot: 100% identical to KITH_SHIBS: Thymidine kinase (tdk) from Shigella boydii serotype 4 (strain Sb227)

KEGG orthology group: K00857, thymidine kinase [EC: 2.7.1.21] (inferred from 99% identity to ebw:BWG_1065)

MetaCyc: 99% identical to thymidine/deoxyuridine kinase (Escherichia coli K-12 substr. MG1655)
Deoxynucleoside kinase. [EC: 2.7.1.145, 2.7.1.21]; 2.7.1.145,2.7.1.21 [EC: 2.7.1.145, 2.7.1.21]

Predicted SEED Role

"Thymidine kinase (EC 2.7.1.21)" in subsystem Folate Biosynthesis (EC 2.7.1.21)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleoside salvage (8/9 steps found)
• pyrimidine deoxyribonucleosides salvage (4/5 steps found)
• purine deoxyribonucleosides salvage (4/6 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.145 or 2.7.1.21

Protein Sequence (205 amino acids)

>OHPLBJKB_02447 Thymidine kinase (Escherichia coli HS(pFamp)R (ATCC 700891))
MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRFGAGKVSSRIGLSSPAK
LFNQNSSLFDEIRSEHEQQAIHCVLVDECQFLTRQQVYELSEVVDQLDIPVLCYGLRTDF
RGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQAGRPYNEGEQVVIGGNERYVS
VCRKHYKEALQVGSLTAIQERHRHD