Protein Info for OHPLBJKB_02313 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Phenylacetate-coenzyme A ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02155: phenylacetate-CoA ligase
  • amino acids 11 to 432 (422 residues), 788.1 bits, E=7.4e-242
• PF00501: AMP-binding
  • amino acids 76 to 289 (214 residues), 64.7 bits, E=7.2e-22
• PF14535: AMP-binding_C_2
  • amino acids 336 to 432 (97 residues), 96.1 bits, E=1.3e-31

Best Hits

Swiss-Prot: 100% identical to PAAK_ECOLI: Phenylacetate-coenzyme A ligase (paaK) from Escherichia coli (strain K12)

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to eco:b1398)

MetaCyc: 66% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30) PaaF" (EC 6.2.1.30)

MetaCyc Pathways

• superpathway of phenylethylamine degradation (11/11 steps found)
• phenylacetate degradation I (aerobic) (9/9 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
• penicillin G and penicillin V biosynthesis (1/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (3/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.30

Protein Sequence (437 amino acids)

>OHPLBJKB_02313 Phenylacetate-coenzyme A ligase (Escherichia coli HS(pFamp)R (ATCC 700891))
MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKFNAAGVHPDDFRELSDL
RKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTVVGYTQNDIDNWANIVARS
LRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIPMSGGQTEKQAQLIRDFQPDM
IMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPWTQAMRKEIERRLGITALDIYGL
SEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDGTPLADGEHGELLFTTLTKEALPVI
RYRTRDLTRLLPGTARTMRRMDRISGRSDDMLIIRGVNVFPSQLEEEIVKFEHLSPHYQL
EVNRRGHLDSLSVKVELKESSLTLTHEQRCQVCHQLRHRIKSMVGISTDVMIVNCGSIPR
SEGKACRVFDLRNIVGA