Protein Info for OHPLBJKB_02138 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: putative dimethyl sulfoxide reductase chain YnfF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 798 signal peptide" amino acids 1 to 37 (37 residues), see Phobius details TIGR02166: anaerobic dimethyl sulfoxide reductase, A subunit, DmsA/YnfE family" amino acids 5 to 798 (794 residues), 1286 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 43 to 102 (60 residues), 47.4 bits, see alignment 2.3e-16 PF00384: Molybdopterin" amino acids 105 to 556 (452 residues), 217.9 bits, see alignment E=3.2e-68 PF01568: Molydop_binding" amino acids 677 to 790 (114 residues), 92.9 bits, see alignment E=2e-30

Best Hits

Swiss-Prot: 100% identical to YNFF_ECOLI: Probable dimethyl sulfoxide reductase chain YnfF (ynfF) from Escherichia coli (strain K12)

KEGG orthology group: K07310, Tat-targeted selenate reductase subunit YnfF [EC: 1.97.1.9] (inferred from 100% identity to ebd:ECBD_2058)

MetaCyc: 71% identical to putative selenate reductase YnfE (Escherichia coli K-12 substr. MG1655)
Selenate reductase. [EC: 1.97.1.9]

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 1.97.1.9

Use Curated BLAST to search for 1.97.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (798 amino acids)

>OHPLBJKB_02138 putative dimethyl sulfoxide reductase chain YnfF (Escherichia coli HS(pFamp)R (ATCC 700891))
MKAEISRRSLMKTSALGSLALASSAFTLPFSQMVRAAEVPVEEKAVWSSCTVNCGSRCLL
RLHVKDDTVYWVESDTTGDDVYGNHQVRACLRGRSIRRRMNHPDRLKYPMKRVGKRGEGK
FERISWDEALDTISDNLRRILKDYGNEAVHVLYGTGVDGGNITNSNVPYRLMNSCGGFLS
RYGSYSTAQISAAMSYMFGANDGNSPDDIANTKLVVMFGNNPAETRMSGGGVTYYVEQAR
ERSNARMIVIDPRYNDTAAGREDEWLPIRPGTDGALACAIAWVLITENMVDQPFLDKYCV
GYDEKTLPANAPRNAHYKAYILGEGPDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPA
YICQGWGPQRHSNGEQTSRAIAMLSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVK
TQISVFTWTDAIDHGTEMTATRDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVL
QDDSKCEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAK
FERKPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGI
FKKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAY
TPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRH
GDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPS
PLAKGNPSHSNLVQIEKV