Protein Info for OHPLBJKB_01896 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Ribosomal RNA small subunit methyltransferase F

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 PF17125: Methyltr_RsmF_N" amino acids 10 to 107 (98 residues), 95.6 bits, see alignment E=4.3e-31 TIGR00446: NOL1/NOP2/sun family putative RNA methylase" amino acids 43 to 312 (270 residues), 387.4 bits, see alignment E=1.5e-120 PF01189: Methyltr_RsmB-F" amino acids 120 to 311 (192 residues), 156.9 bits, see alignment E=1.1e-49 PF21150: YebU_pre-PUA_dom" amino acids 331 to 407 (77 residues), 128.3 bits, see alignment E=1.7e-41 PF13636: Methyltranf_PUA" amino acids 423 to 471 (49 residues), 43.3 bits, see alignment 6.2e-15

Best Hits

Swiss-Prot: 100% identical to RSMF_ECOLC: Ribosomal RNA small subunit methyltransferase F (rsmF) from Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks)

KEGG orthology group: K11392, ribosomal RNA small subunit methyltransferase F [EC: 2.1.1.-] (inferred from 100% identity to ecx:EcHS_A1926)

MetaCyc: 98% identical to 16S rRNA m5C1407 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-11593 [EC: 2.1.1.178]

Predicted SEED Role

"Ribosomal RNA small subunit methyltransferase F (EC 2.1.1.-)" (EC 2.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.- or 2.1.1.178

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (481 amino acids)

>OHPLBJKB_01896 Ribosomal RNA small subunit methyltransferase F (Escherichia coli HS(pFamp)R (ATCC 700891))
MLVAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTA
PYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADGNAP
QRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGR
VFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRP
GGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGELFPAANKALTEEGFLHVFPQIY
DCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKDREAGQIRQAAAGVGLNWDENLRLW
QRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALATPDNVNAFELTP
QEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTSN
A