Protein Info for OHPLBJKB_01669 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Vitamin B12 import ATP-binding protein BtuD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 431 PF00005: ABC_tran" amino acids 40 to 172 (133 residues), 81.7 bits, see alignment E=8.1e-27 PF14524: Wzt_C" amino acids 283 to 423 (141 residues), 89.7 bits, see alignment E=1.8e-29

Best Hits

KEGG orthology group: K09691, lipopolysaccharide transport system ATP-binding protein (inferred from 100% identity to ecx:EcHS_A2170)

MetaCyc: 99% identical to O:9 antigen ABC transporter ATP-binding component (Escherichia coli O9a)
RXN-22100 [EC: 7.5.2.14]

Predicted SEED Role

"Teichoic acid export ATP-binding protein TagH (EC 3.6.3.40)" in subsystem Rhamnose containing glycans or Teichoic and lipoteichoic acids biosynthesis (EC 3.6.3.40)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.3.40 or 7.5.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (431 amino acids)

>OHPLBJKB_01669 Vitamin B12 import ATP-binding protein BtuD (Escherichia coli HS(pFamp)R (ATCC 700891))
MSIKVQHVGKAYKYYPSKWNRVIEKLLPGDKPRHSKKWVLKDINFSIEPGEAVGIVGVNG
AGKSTLLKLLTGTTQPTKGSIEIQGRVAALLELGMGFHPDFTGRQNVYMSGLMMGLGREE
IERLMPEIEAFADIGDYIEEPVRIYSSGMQMRLAFAVATASRPDILIVDEALSVGDSRFQ
AKCYARIADFKKQGTTLLLVSHSAGDIVKHCDRAIFLKNGDICMDGTARDVTNRYLDELF
GKADKNSAPKSETATSSASGESQMSLDEIEDVYHTRPGYRPEEYRWGQGGAKIIDYHIQS
AGVDFPPSLTGNQQTDFLMKVVFEYDFDCVVPGILIKTLDGLFLYGTNSFLASEGRENIS
VSRGDVRVFKFSLPVDLNSGDYLLSFGISAGNPQTDMTPLDRRYDSIILHVTKSMDFWGV
IDLKSSFTSYQ