Protein Info for OHPLBJKB_01549 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Pyrimidine-specific ribonucleoside hydrolase RihB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 6 to 299 (294 residues), 330.9 bits, E=4.8e-103

Best Hits

Swiss-Prot: 100% identical to RIHB_ECO8A: Pyrimidine-specific ribonucleoside hydrolase RihB (rihB) from Escherichia coli O8 (strain IAI1)

KEGG orthology group: K10213, ribosylpyrimidine nucleosidase [EC: 3.2.2.8] (inferred from 99% identity to eco:b2162)

MetaCyc: 99% identical to pyrimidine-specific ribonucleoside hydrolase RihB (Escherichia coli K-12 substr. MG1655)
Ribosylpyrimidine nucleosidase. [EC: 3.2.2.8]; Uridine nucleosidase. [EC: 3.2.2.8, 3.2.2.3]; 3.2.2.3 [EC: 3.2.2.8, 3.2.2.3]

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• superpathway of pyrimidine ribonucleosides salvage (10/10 steps found)
• purine nucleotides degradation I (plants) (10/12 steps found)
• adenosine nucleotides degradation I (7/8 steps found)
• guanosine nucleotides degradation II (4/4 steps found)
• superpathway of guanosine nucleotides degradation (plants) (5/6 steps found)
• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• guanosine nucleotides degradation I (3/4 steps found)
• superpathway of purines degradation in plants (13/18 steps found)
• NAD salvage (plants) (5/11 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.1

Use Curated BLAST to search for 3.2.2.1 or 3.2.2.3 or 3.2.2.8

Protein Sequence (313 amino acids)

>OHPLBJKB_01549 Pyrimidine-specific ribonucleoside hydrolase RihB (Escherichia coli HS(pFamp)R (ATCC 700891))
MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLDKTLINGLNVCQKLEIN
VPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAESTHAVKYIIDTLMASDGDIT
LVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEAARVVFTS
GVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENYGLAGGPVHD
ATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKVGITIDTDWFWG
LVEECVRGYIKTH