Protein Info for OHPLBJKB_01456 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Bifunctional polymyxin resistance protein ArnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 660 PF00551: Formyl_trans_N" amino acids 47 to 176 (130 residues), 86.5 bits, see alignment E=5.1e-28 PF02911: Formyl_trans_C" amino acids 202 to 294 (93 residues), 77 bits, see alignment E=2.8e-25 PF01370: Epimerase" amino acids 318 to 566 (249 residues), 158.7 bits, see alignment E=4.4e-50 PF16363: GDP_Man_Dehyd" amino acids 319 to 645 (327 residues), 100.3 bits, see alignment E=4.1e-32 PF01073: 3Beta_HSD" amino acids 319 to 475 (157 residues), 23.1 bits, see alignment E=8.5e-09

Best Hits

Swiss-Prot: 100% identical to ARNA_ECOHS: Bifunctional polymyxin resistance protein ArnA (arnA) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K10011, UDP-4-amino-4-deoxy-L-arabinose formyltransferase / UDP-glucuronic acid dehydrogenase (UDP-4-keto-hexauronic acid decarboxylating) [EC: 1.1.1.305 2.1.2.13] (inferred from 100% identity to ecx:EcHS_A2400)

MetaCyc: 99% identical to fused UDP-4-amino-4-deoxy-L-arabinose formyltransferase/UDP-glucuronate dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN0-1862 [EC: 2.1.2.13]; RXN0-1861 [EC: 2.1.2.13, 1.1.1.305]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.305 or 2.1.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (660 amino acids)

>OHPLBJKB_01456 Bifunctional polymyxin resistance protein ArnA (Escherichia coli HS(pFamp)R (ATCC 700891))
MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGSVAHLAAERGIPVYAPD
NVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFNLHGSLLPKYRGRAPLNWV
LVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAITLHHKLCHAARQLLEQTLPAI
KHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLHNMVRAVADPWPGAFSYVGNQKF
TVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEIVTGQAGDGITMQGSQLAQTLGLVQ
GSRLNSQPACTARRRTRVLILGVNGFIGNHLTERLLREDHYEVYGLDIGSDAISRFLNHP
HFHFVEGDISIHSEWIEYHVKKCDVVLPLVAIATPIEYTRNPLRVFELDFEENLRIIRYC
VKYRKRIIFPSTSEVYGMCSDKYFDEDHSNLIVGPVNKPRWIYSVSKQLLDRVIWAYGEK
EGLQFTLFRPFNWMGPRLDNLNAARIGSSRAITQLILNLVEGSPIKLIDGGKQKRCFTDI
RDGIEALYRIIENAGNRCDGEIINIGNPENEASIEELGEMLLASFEKHPLRHHFPPFAGF
RVVESSSYYGKGYQDVEHRKPSIRNAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS