Protein Info for OHPLBJKB_01239 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Hydrogenase-4 component G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 555 PF00329: Complex1_30kDa" amino acids 39 to 155 (117 residues), 85.7 bits, see alignment E=5.9e-28 PF00374: NiFeSe_Hases" amino acids 220 to 288 (69 residues), 48 bits, see alignment E=1.5e-16 PF00346: Complex1_49kDa" amino acids 297 to 447 (151 residues), 77.3 bits, see alignment E=1.6e-25 amino acids 445 to 523 (79 residues), 42.6 bits, see alignment E=6.1e-15

Best Hits

Swiss-Prot: 100% identical to HYFG_ECOLI: Hydrogenase-4 component G (hyfG) from Escherichia coli (strain K12)

KEGG orthology group: K12142, hydrogenase-4 component G [EC: 1.-.-.-] (inferred from 100% identity to eco:b2487)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (555 amino acids)

>OHPLBJKB_01239 Hydrogenase-4 component G (Escherichia coli HS(pFamp)R (ATCC 700891))
MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINLLPDVVQYLYYQHDGWL
PVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSREFPSVTPRVPAAVWGERE
IRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRLRPEPTTDSETYPFINEGNSD
ARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYVHRGMEKLAETRMGYNEVTFLSD
RVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSILLEVERLHSHLLNLGLSCHFVGFD
TGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGGVRRDILKEQRLQTLKLVREMRADVSE
LVEMLLATPNMEQRTQGIGILDRQIARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVR
VKETFDSLAMLEFALDNMQDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLF
RWRCRAATYANWPVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYK
EIERYGIDRNRSPLK