Protein Info for OHPLBJKB_01198 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: GTPase Der

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03594: ribosome-associated GTPase EngA
  • amino acids 3 to 458 (456 residues), 596.1 bits, E=6.4e-183
• TIGR00231: small GTP-binding protein domain
  • amino acids 4 to 157 (154 residues), 74.8 bits, E=1e-24
  • amino acids 203 to 368 (166 residues), 83.5 bits, E=2.2e-27
• PF02421: FeoB_N
  • amino acids 5 to 156 (152 residues), 58 bits, E=3.7e-19
  • amino acids 204 to 366 (163 residues), 47.4 bits, E=7e-16
• PF01926: MMR_HSR1
  • amino acids 5 to 119 (115 residues), 106.7 bits, E=3.4e-34
  • amino acids 205 to 322 (118 residues), 96.6 bits, E=4.6e-31
• PF00009: GTP_EFTU
  • amino acids 204 to 370 (167 residues), 42.9 bits, E=1.9e-14
• PF14714: KH_dom-like
  • amino acids 378 to 458 (81 residues), 95 bits, E=1.2e-30

Best Hits

Swiss-Prot: 100% identical to DER_ECO24: GTPase Der (der) from Escherichia coli O139:H28 (strain E24377A / ETEC)

KEGG orthology group: K03977, GTP-binding protein (inferred from 100% identity to eco:b2511)

MetaCyc: 100% identical to 50S ribosomal subunit stability factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (490 amino acids)

>OHPLBJKB_01198 GTPase Der (Escherichia coli HS(pFamp)R (ATCC 700891))
MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGRAEIEGREFICIDTGGI
DGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAIAKHLRSREKPTFLVANKT
DGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLLPWMEDLAPQEEVDEDAEYWA
QFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDS
IYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVIKTLQAIEDANVVMLVIDAREGISD
QDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQVKETLDFRLGFIDFARVHFISALHGSG
VGNLFESVREAYDSSTRRVGTSMLTRIMTMAVEDHQPPLVRGRRVKLKYAHAGGYNPPIV
VIHGNQVKDLPDSYKRYLMNYFRKSLDVMGSPIRIQFKEGENPYANKRNTLTPTQMRKRK
RLMKHIKKNK