Protein Info for OHPLBJKB_01141 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: GTPase Era

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00231: small GTP-binding protein domain
  • amino acids 7 to 170 (164 residues), 140.1 bits, E=5.8e-45
• TIGR00436: GTP-binding protein Era
  • amino acids 9 to 280 (272 residues), 400.1 bits, E=4.4e-124
• PF01926: MMR_HSR1
  • amino acids 11 to 125 (115 residues), 90.7 bits, E=2.8e-29
• PF02421: FeoB_N
  • amino acids 11 to 166 (156 residues), 50.7 bits, E=5.9e-17
• PF04548: AIG1
  • amino acids 11 to 114 (104 residues), 27.7 bits, E=6.5e-10
• PF00009: GTP_EFTU
  • amino acids 11 to 174 (164 residues), 32.9 bits, E=2e-11
• PF07650: KH_2
  • amino acids 209 to 285 (77 residues), 72.1 bits, E=1.1e-23

Best Hits

Swiss-Prot: 100% identical to ERA_ECOLI: GTPase Era (era) from Escherichia coli (strain K12)

KEGG orthology group: K03595, GTP-binding protein Era (inferred from 100% identity to eco:b2566)

MetaCyc: 100% identical to 30S ribosomal subunit maturation GTPase Era (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein Era" in subsystem Bacterial Cell Division or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (301 amino acids)

>OHPLBJKB_01141 GTPase Era (Escherichia coli HS(pFamp)R (ATCC 700891))
MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTRHRIVGIHTEGAYQAIY
VDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWTPDDEMVLNKLREGKAPVI
LAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGLNVDTIAAIVRKHLPEATHHF
PEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVERE
GQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYVDD
L