Protein Info for OHPLBJKB_00814 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: 6-phospho-beta-glucosidase BglA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF00232: Glyco_hydro_1" amino acids 5 to 474 (470 residues), 436.1 bits, see alignment E=6.3e-135

Best Hits

Swiss-Prot: 100% identical to BGLA_ECOLI: 6-phospho-beta-glucosidase BglA (bglA) from Escherichia coli (strain K12)

KEGG orthology group: K01223, 6-phospho-beta-glucosidase [EC: 3.2.1.86] (inferred from 100% identity to eco:b2901)

MetaCyc: 100% identical to 6-phospho-beta-glucosidase A (Escherichia coli K-12 substr. MG1655)
3.2.1.M25 [EC: 3.2.1.M25]; 6-phospho-beta-glucosidase. [EC: 3.2.1.M25, 3.2.1.86]

Predicted SEED Role

"6-phospho-beta-glucosidase (EC 3.2.1.86)" in subsystem Beta-Glucoside Metabolism (EC 3.2.1.86)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.86

Use Curated BLAST to search for 3.2.1.86 or 3.2.1.M25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (477 amino acids)

>OHPLBJKB_00814 6-phospho-beta-glucosidase BglA (Escherichia coli HS(pFamp)R (ATCC 700891))
MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHGVPREITKEVLPGKYYP
NHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEAQPNEEGLKFYDDMFDELL
KYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAEVVFERYKHKVKYWMTFNEIN
NQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFVASALAVKAARRINPEMKVGCML
AMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGYYPSYVLNEWERRGFNIKMEDGDLD
VLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFEGSVPNPYVKASDWGWQIDPVGLRYAL
CELYERYQRPLFIVENGFGAYDKVEEDGSINDDYRIDYLRAHIEEMKKAVTYDGVDLMGY
TPWGCIDCVSFTTGQYSKRYGFIYVNKHDDGTGDMSRSRKKSFNWYKEVIASNGEKL