Protein Info for OHPLBJKB_00666 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Putative acid--amine ligase YgiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03738: GSP_synth
  • amino acids 12 to 384 (373 residues), 549.3 bits, E=2.4e-169

Best Hits

Swiss-Prot: 100% identical to YGIC_ECOL6: Putative acid--amine ligase YgiC (ygiC) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 100% identity to eco:b3038)

MetaCyc: 100% identical to peptide--spermidine ligase YgiC (Escherichia coli K-12 substr. MG1655)
6.3.1.-

Predicted SEED Role

"Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1" in subsystem Glutathionylspermidine and Trypanothione (EC 6.3.1.8)

MetaCyc Pathways

• glutathionylspermidine biosynthesis (1/1 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.8

Use Curated BLAST to search for 6.3.1.8

Protein Sequence (386 amino acids)

>OHPLBJKB_00666 Putative acid--amine ligase YgiC (Escherichia coli HS(pFamp)R (ATCC 700891))
MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQVEKLEEVTAELHQMCL
KVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLDLAWDGTGEPKLLENNADT
PTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLIDRFVELREQYGFQLLHLTCCR
DTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQFTDLQDQVISNLFKLYPWEFML
REMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEMFPNHPNLLPAYFAEDDHPQMEKYV
VKPIFSREGANVSIIENGKTIEAAEGPYGEEGMIVQQFHPLPKFGDSYMLIGSWLVNDQP
AGIGIREDRALITQDMSRFYPHIFVE