Protein Info for OHPLBJKB_00622 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Glucosidase YgjK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 783 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF21152: YgjK_N" amino acids 33 to 286 (254 residues), 430.1 bits, see alignment E=5.6e-133 PF01204: Trehalase" amino acids 414 to 761 (348 residues), 42.3 bits, see alignment E=9.8e-15

Best Hits

Swiss-Prot: 100% identical to YGJK_ECOLI: Glucosidase YgjK (ygjK) from Escherichia coli (strain K12)

KEGG orthology group: K03931, putative isomerase (inferred from 100% identity to eco:b3080)

MetaCyc: 100% identical to glycoside hydrolase (Escherichia coli K-12 substr. MG1655)
3.2.1.-

Predicted SEED Role

"Putative isomerase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (783 amino acids)

>OHPLBJKB_00622 Glucosidase YgjK (Escherichia coli HS(pFamp)R (ATCC 700891))
MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDYDYDDHQRFNPFFDLGA
WHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQDGKKVDFTLEAYSIPGALV
QKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDGELLEKLEAKEGKPLSDKTIA
GEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEYQVHKSLPVQTEINGNRFTSKAH
INGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAFYLTASQQRWEEYLKKGLTNPDATP
EQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVTGRWFSGNQTWPWDTWKQAFAMAHFNP
DIAKENIRAVFSWQIQPGDSVRPQDVGFVPDLIAWNLSPERGGDGGNWNERNTKPSLAAW
SVMEVYNVTQDKTWLAELYPKLVAYHDWWLRNRDHNGNGVPEYGATRDKAHNTESGEMLF
TVKKGDKEETQSGLNNYARVVEKGQYDSLEIPAQVAASWESGRDDAAVFGFIDKEQLDKY
VANGGKRSDWTVKFAENRSQDGTLLGYSLLQESVDQASYMYSDNHYLAEMATILGKPEEA
KRYRQLAQQLADYINTCMFDPTTQFYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFN
GAATQANADAVVKVMLDPKEFNTFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGME
RYGYRDDALKLADTFFRHAKGLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFF
RKQ