Protein Info for OHPLBJKB_00470 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: Periplasmic pH-dependent serine endoprotease DegQ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details TIGR02037: peptidase Do" amino acids 41 to 453 (413 residues), 532.2 bits, see alignment E=5.1e-164 PF13365: Trypsin_2" amino acids 92 to 228 (137 residues), 114.6 bits, see alignment E=2e-36 PF00089: Trypsin" amino acids 93 to 251 (159 residues), 72.6 bits, see alignment E=1.3e-23 PF00595: PDZ" amino acids 261 to 345 (85 residues), 59.1 bits, see alignment E=1.4e-19 amino acids 371 to 443 (73 residues), 49.9 bits, see alignment E=1.1e-16 PF13180: PDZ_2" amino acids 287 to 358 (72 residues), 56.6 bits, see alignment E=8.1e-19 amino acids 386 to 447 (62 residues), 32.5 bits, see alignment E=2.6e-11 PF17820: PDZ_6" amino acids 293 to 347 (55 residues), 36.5 bits, see alignment 1e-12 amino acids 394 to 444 (51 residues), 34.2 bits, see alignment 5.3e-12

Best Hits

Swiss-Prot: 100% identical to DEGQ_ECOLI: Periplasmic pH-dependent serine endoprotease DegQ (degQ) from Escherichia coli (strain K12)

KEGG orthology group: K04772, serine protease DegQ [EC: 3.4.21.-] (inferred from 100% identity to eco:b3234)

MetaCyc: 100% identical to periplasmic serine endoprotease (Escherichia coli K-12 substr. MG1655)
3.4.21.-

Predicted SEED Role

"Outer membrane stress sensor protease DegQ, serine protease" in subsystem Proteolysis in bacteria, ATP-dependent

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>OHPLBJKB_00470 Periplasmic pH-dependent serine endoprotease DegQ (Escherichia coli HS(pFamp)R (ATCC 700891))
MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAPMLEKVLPAVVSVRVEG
TASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKGYVLTNNHVINQAQKISIQ
LNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSDKLRVGDFAVAVGNPFGLGQT
ATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGALLNLNGELIGINTAILAPGGGSV
GIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEMSADIAKAFNLDVQRGAFVSEVLPG
SGSAKAGVKAGDIITSLNGKPLNSFAELRSRIATTEPGTKVKLGLLRNGKPLEVEVTLDT
STSSSASAEMITPALEGATLSDGQLKDGGKGIKIDEVVKGSPAAQAGLQKDDVIIGVNRD
RVNSIAEMRKVLAAKPAIIALQIVRGNESIYLLMR