Protein Info for OHPLBJKB_00269 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: 1,4-alpha-glucan branching enzyme GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01515: 1,4-alpha-glucan branching enzyme
  • amino acids 109 to 723 (615 residues), 1118.6 bits, E=0
• PF02922: CBM_48
  • amino acids 125 to 207 (83 residues), 95.2 bits, E=3.6e-31
• PF00128: Alpha-amylase
  • amino acids 273 to 344 (72 residues), 32 bits, E=1.5e-11
• PF02806: Alpha-amylase_C
  • amino acids 628 to 725 (98 residues), 92.2 bits, E=3.2e-30

Best Hits

Swiss-Prot: 100% identical to GLGB_ECOLI: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Escherichia coli (strain K12)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to ebw:BWG_3124)

MetaCyc: 100% identical to 1,4-alpha-glucan branching enzyme (Escherichia coli K-12 substr. MG1655)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

• glycogen biosynthesis I (from ADP-D-Glucose) (4/4 steps found)
• starch biosynthesis (6/10 steps found)
• glycogen biosynthesis II (from UDP-D-Glucose) (1/4 steps found)
• glycogen biosynthesis III (from α-maltose 1-phosphate) (3/8 steps found)

KEGG Metabolic Maps

• Starch and sucrose metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Protein Sequence (728 amino acids)

>OHPLBJKB_00269 1,4-alpha-glucan branching enzyme GlgB (Escherichia coli HS(pFamp)R (ATCC 700891))
MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDATDVWVIEPKTGRKLAKL
ECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFGPLIQEMDAWLLSEGTHLR
PYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPMRLRKESGIWELFIP
GAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETASLICGLPEKVVQTEERKKANQFD
APISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYAKWMGFTHLELLPINEHPFDGSWGY
QPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHS
DPREGYHQDWNTLIYNYGRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSRKEG
EWIPNEFGGRENLEAIEFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGFWYK
WNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDEVVHGKKSILDRMP
GDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQ
RLVRDLNLTYRHHKAMHELDFDPYGFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPV
PRHDYRFGINQPGKWREILNTDSMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLAT
IWLVREAE