Protein Info for OHPLBJKB_00078 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: O-antigen ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 417 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details amino acids 36 to 51 (16 residues), see Phobius details amino acids 63 to 80 (18 residues), see Phobius details amino acids 100 to 117 (18 residues), see Phobius details amino acids 129 to 147 (19 residues), see Phobius details amino acids 166 to 183 (18 residues), see Phobius details amino acids 191 to 207 (17 residues), see Phobius details amino acids 213 to 231 (19 residues), see Phobius details amino acids 238 to 255 (18 residues), see Phobius details amino acids 342 to 361 (20 residues), see Phobius details amino acids 368 to 386 (19 residues), see Phobius details amino acids 392 to 408 (17 residues), see Phobius details PF04932: Wzy_C" amino acids 197 to 349 (153 residues), 67.9 bits, see alignment E=4.4e-23

Best Hits

KEGG orthology group: K02847, O-antigen ligase [EC: 6.-.-.-] (inferred from 100% identity to ecx:EcHS_A3833)

Predicted SEED Role

"O-antigen ligase" in subsystem LOS core oligosaccharide biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.-.-.-

Use Curated BLAST to search for 6.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (417 amino acids)

>OHPLBJKB_00078 O-antigen ligase (Escherichia coli HS(pFamp)R (ATCC 700891))
MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGIISALYFLVTPKKTITNN
PTLLIFISLCLLGIVNIIWYSHYKISGSVYTNAYRGPMETGKIALCSAFIFLVLFAKNEM
RTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASI
LILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLY
CFITIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQ
SLEKRAEKIHELEEKEPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYA
LRTAKEPYILILLFSLLVVGLSDVILFSKPVPTAVFITIILLCAYFKAQSDQCLLEK