Protein Info for OHPLBJKB_00054 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: DNA ligase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01653: DNA_ligase_aden" amino acids 115 to 306 (192 residues), 80.6 bits, see alignment E=1.2e-26 PF03120: DNA_ligase_OB" amino acids 312 to 386 (75 residues), 74.2 bits, see alignment E=6.3e-25

Best Hits

Swiss-Prot: 100% identical to LIGB_ECOHS: DNA ligase B (ligB) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to eco:b3647)

MetaCyc: 100% identical to DNA ligase B (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2), LigB" (EC 6.5.1.2)

Isozymes

Compare fitness of predicted isozymes for: 6.5.1.2

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (560 amino acids)

>OHPLBJKB_00054 DNA ligase B (Escherichia coli HS(pFamp)R (ATCC 700891))
MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDVYWKEGKSEVEDGV
YDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLW
VQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGE
IFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMSDRLKELA
TAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVTDGVVVRAAKEPESRHWLPGQAEWL
VAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIA
PGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWL
GAKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKEN
AQIKKLGSWLAAQQITGFEP