Protein Info for OHPLBJKB_00003 in Escherichia coli HS(pFamp)R (ATCC 700891)

Annotation: DNA replication and repair protein RecF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 357 PF13514: AAA_27" amino acids 1 to 93 (93 residues), 24.3 bits, see alignment E=5.3e-09 TIGR00611: DNA replication and repair protein RecF" amino acids 1 to 355 (355 residues), 467.4 bits, see alignment E=1.7e-144 PF13175: AAA_15" amino acids 1 to 46 (46 residues), 37.9 bits, see alignment 4.2e-13 PF02463: SMC_N" amino acids 3 to 355 (353 residues), 165.9 bits, see alignment E=2.5e-52 PF13476: AAA_23" amino acids 5 to 44 (40 residues), 33.4 bits, see alignment 1.8e-11 PF13304: AAA_21" amino acids 25 to 47 (23 residues), 27.3 bits, see alignment (E = 8.6e-10)

Best Hits

Swiss-Prot: 100% identical to RECF_ECO24: DNA replication and repair protein RecF (recF) from Escherichia coli O139:H28 (strain E24377A / ETEC)

KEGG orthology group: K03629, DNA replication and repair protein RecF (inferred from 100% identity to eco:b3700)

MetaCyc: 100% identical to recombination mediator protein RecF (Escherichia coli K-12 substr. MG1655)
RXN0-2606

Predicted SEED Role

"DNA recombination and repair protein RecF" in subsystem DNA repair, bacterial RecFOR pathway

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (357 amino acids)

>OHPLBJKB_00003 DNA replication and repair protein RecF (Escherichia coli HS(pFamp)R (ATCC 700891))
MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRV
IRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPE
GFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKE
LIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFER
DRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYL
IDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD