Protein Info for OH686_20985 in Pseudomonas sp. S08-1

Annotation: asparagine synthase (glutamine-hydrolyzing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03104: asparagine synthase family amidotransferase
  • amino acids 1 to 586 (586 residues), 956.7 bits, E=3.8e-292
• TIGR01536: asparagine synthase (glutamine-hydrolyzing)
  • amino acids 2 to 519 (518 residues), 397.3 bits, E=1.4e-122
• PF13522: GATase_6
  • amino acids 32 to 160 (129 residues), 141.3 bits, E=2.7e-45
• PF13537: GATase_7
  • amino acids 47 to 167 (121 residues), 146.7 bits, E=4.9e-47
• PF00733: Asn_synthase
  • amino acids 245 to 585 (341 residues), 279.8 bits, E=7.5e-87

Best Hits

KEGG orthology group: K01953, asparagine synthase (glutamine-hydrolysing) [EC: 6.3.5.4] (inferred from 81% identity to pfl:PFL_4333)

MetaCyc: 55% identical to N-acetylglutaminylglutamine amide synthase (Sinorhizobium meliloti 1021)
RXN-13456

Predicted SEED Role

"Asparagine synthetase [glutamine-hydrolyzing] (EC 6.3.5.4)" in subsystem Cyanophycin Metabolism or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 6.3.5.4)

MetaCyc Pathways

• L-citrulline biosynthesis (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• L-glutamate and L-glutamine biosynthesis (6/7 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• L-asparagine biosynthesis I (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• N-acetylglutaminylglutamine amide biosynthesis (1/2 steps found)
• superpathway of L-asparagine biosynthesis (1/2 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.4

Protein Sequence (589 amino acids)

>OH686_20985 asparagine synthase (glutamine-hydrolyzing) (Pseudomonas sp. S08-1)
MCGIAGELRFDHQPADLAALARITHHLAPRGPDACGFHSSGSLGLGHRRLKVMDLAPASG
QPMVDQQLGLAMVFNGAIYNYPELRDELRALGYQFHSDGDTEVLLKGYHAWGEALLPKLN
GMFAFAIWERDTERLFLARDRLGVKPLYLSDDGTRLRFASSLPALLQGRDIDSALDPVAL
DHYLNFHAVVPAPRTLLAGVKKLPPATWLRIERDGRRVEHRWWQLDFGPRADESGLQLED
WSERVLDGLRQAVQVRQRAALDVGVLLSGGLDSSLLVGLLHEAGVADLATFSIGFEDAGG
ERGDEFHYSDLIARHYGTRHQQLRIAEAEILAELPDAFRAMSEPMVSHDCIAFYLLGREV
AKHCTVVQCGQGADELFAGYHWYPQVDGAADPQAAYRAAFFDRSREETLATLRPQWHGPD
WAGEFLREHFAMPGAEAAVDKALRLDGTVMLVDDPVKRVDNMTMAWGLEARTPFLDYRLV
ELAARIPARFKLPDGGKYVLKEAGRKVIPHAVIDRPKGYFPVPGLKHLQGATLDWVQELL
LDPSQDRGLFEPAMLDRLLSEPDGQLTPLGGSKLWQLAALNLWLSEQGL