Protein Info for OH686_20375 in Pseudomonas sp. S08-1

Annotation: glycerol kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 TIGR01311: glycerol kinase" amino acids 3 to 492 (490 residues), 728.1 bits, see alignment E=2e-223 PF00370: FGGY_N" amino acids 4 to 250 (247 residues), 249.9 bits, see alignment E=2.8e-78 PF02782: FGGY_C" amino acids 261 to 447 (187 residues), 149 bits, see alignment E=1.6e-47

Best Hits

Swiss-Prot: 87% identical to GLPK_PSEMY: Glycerol kinase (glpK) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 87% identity to pmy:Pmen_3345)

MetaCyc: 55% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.30

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (495 amino acids)

>OH686_20375 glycerol kinase (Pseudomonas sp. S08-1)
MSQYLLAIDQGTTSSRAIVFSAQGLPLASAQQEFKQYFPKDGWVEHDAEEIWLTTLKVCR
EALASKGLKADEVLAIGITNQRETTVVWDAVSGSPIHPAIVWQDRRTADYCAGLKAEGHE
AKVADKTGLLIDPYFSATKLRWILDNVPGARERAERGELRFGTVDCFLLWRLTDGKSHRT
DASNASRTLLFNIHSQQWDDELLQLFDIPRSLLPEVLDCAADFGVTEPALLGAGIPVLGM
AGDQQAALIGQTCFEPGMVKSTYGTGCFMIQNTGDKPVVSKNRLLTTVGYRLNGKVTYAV
EGSIFVAGAAVQWLRDGIKLISDARDTELLAEQTGDACGVYLVPAFTGLGAPYWDPKARG
AIFGLTRDTGIKEIVTAGLQSVCYQTRDLLQAMRQDGAAEPSALRVDGGMVVNNWVMQFL
ADILGVPVERPVVTETTALGVAYLAGLQAGLYRDLDEVASHWHRQQRFAPRMADAHRGKL
YAGWLDAVKRVRSEG