Protein Info for OH686_18910 in Pseudomonas sp. S08-1

Annotation: D-cysteine desulfhydrase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01275: pyridoxal phosphate-dependent enzymes, D-cysteine desulfhydrase family
  • amino acids 45 to 362 (318 residues), 363.6 bits, E=4.8e-113
• PF00291: PALP
  • amino acids 50 to 352 (303 residues), 181.2 bits, E=1.5e-57

Best Hits

Swiss-Prot: 66% identical to DCYD_SERP5: D-cysteine desulfhydrase (dcyD) from Serratia proteamaculans (strain 568)

KEGG orthology group: K05396, D-cysteine desulfhydrase [EC: 4.4.1.15] (inferred from 83% identity to psa:PST_2349)

MetaCyc: 63% identical to D-cysteine desulfhydrase (Escherichia coli K-12 substr. MG1655)
3-chloro-D-alanine dehydrochlorinase. [EC: 4.5.1.2]; D-cysteine desulfhydrase. [EC: 4.5.1.2, 4.4.1.15]

Predicted SEED Role

"D-cysteine desulfhydrase (EC 4.4.1.15)" (EC 4.4.1.15)

MetaCyc Pathways

• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-serine degradation (3/3 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• D-serine degradation (2/3 steps found)
• L-cysteine degradation II (2/3 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• glycine betaine degradation I (5/8 steps found)
• glycine betaine degradation III (4/7 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• purine nucleobases degradation II (anaerobic) (10/24 steps found)

KEGG Metabolic Maps

• Cysteine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.4.1.15 or 4.5.1.2

Protein Sequence (366 amino acids)

>OH686_18910 D-cysteine desulfhydrase  (Pseudomonas sp. S08-1)
MGKPVEAFSFVQRNCRALAQLTRVTTFHSGLPNMLRQALARFPRLELIPSATPLEHLPRL
SRHLGRDIWIKRDDLTPLALGGNKARKLEFLAADALAQGADVLVTAGAIQSNHVRQTAAI
AARLGLGCLALLENPIGTADSNYLSNGNRLLLDLFGAEIEAVDNLDNADQLLADACERLR
AAGRKPYSVPIGGSNALGALGYVRAGLELAEQIQHTNQDFAAVLLASGSAGTHSGLALAL
EQTRPGTRVVGVTVSRPDATQRPKVESLLQRTADLLEVNVPAGLRIELWDQYFAPRYGEP
NAGTLEAIQLLARLEGVLLDPVYTGKAFAGLLDGLAQGAFPDDGPLLFLHTGGAPALFAY
HPAGPA