Protein Info for OH686_18010 in Pseudomonas sp. S08-1

Annotation: malate dehydrogenase (acceptor)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 8 to 494 (487 residues), 755.5 bits, E=3.5e-231
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 9 to 492 (484 residues), 797.3 bits, E=2.5e-244
• PF01266: DAO
  • amino acids 10 to 313 (304 residues), 36.9 bits, E=5e-13
• PF01494: FAD_binding_3
  • amino acids 154 to 258 (105 residues), 23.3 bits, E=5.3e-09

Best Hits

Swiss-Prot: 78% identical to MQO2_PSEAE: Probable malate:quinone oxidoreductase 2 (mqo2) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 78% identity to pau:PA14_61400)

MetaCyc: 52% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.5.4

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (495 amino acids)

>OH686_18010 malate dehydrogenase (acceptor) (Pseudomonas sp. S08-1)
MTQVNHETVDVLLVGAGIMSATLAVLLKELDPNLKLEIAELRSSGAIESSNPWNNAGTGH
AGLCELNYTPQAADGSVDIKKAVQINTQFEVSKQFWSYLCGKSAFGSPKNFINPVPHLSF
VRGEKDIGFLKTRHAALTQHHAFAGMEYTEDRAAMAEWMPLMMQGRDANERIAATRALNG
TDVNFGALTNQLLQYLAQQGGTTVSYSQKVTGLKRTADGWSAEVKNTKSGEQREIKAKFV
FLGAGGGALPLLQMSGIEEGKGFGGFPVSGQWLRCDNPEVVKQHQAKVYSQAEVGAPPMS
VPHLDTRVVDGKTSLLFGPYAGFTTKFLKYGSFLDLPLSVRPSNIGPMLAVARDNFDLTR
YLIKEVLQSPEQRLDSLRKFYPNLNPADWRLEIAGQRVQIIKKDAKKGGILQFGTELVAA
EDGTIAALLGASPGASVTVSIMLNLIERCFAAQAGSAAWAAKLKEIFPAREKVLEEDAAL
YRSVSEQSDKALQLV