Protein Info for OH686_16860 in Pseudomonas sp. S08-1

Annotation: Arylsulfatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 537 PF00884: Sulfatase" amino acids 5 to 421 (417 residues), 221 bits, see alignment E=2.3e-69 PF16347: SGSH_C" amino acids 375 to 507 (133 residues), 37.7 bits, see alignment E=2.4e-13

Best Hits

Swiss-Prot: 83% identical to ARS_PSEAE: Arylsulfatase (atsA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01130, arylsulfatase [EC: 3.1.6.1] (inferred from 83% identity to pag:PLES_01841)

MetaCyc: 83% identical to arylsulfatase (Pseudomonas aeruginosa)
Arylsulfatase. [EC: 3.1.6.1]; 3.1.6.1 [EC: 3.1.6.1]

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.6

Use Curated BLAST to search for 3.1.6.1 or 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (537 amino acids)

>OH686_16860 Arylsulfatase  (Pseudomonas sp. S08-1)
MSKRPNFLLIVADDLGFSDLGAFGGEIATPNLDALALDGLRLTDFHVAPTCSPTRSMLLT
GTDHHIAGIGSMAEAIAPEQIGQPGYEGYLNERVVALPELLREAGYQTLMAGKWHLGLTP
ELAPHARGFERSFSLLPGAANHYGFEPPYDESTPGILKSTPALYIEDDRFVEQLPEGFYS
SDAFGDRLIQYLKERDQARPFFAYLPFSAPHWPLQAPQEVVAKYRGRYDAGPEALRQERL
QRLKQLGLVAEDVQAHPVLALTQEWERLTPQQRAISARTMEVYAAMVERMDWNIGRVVDY
LRRQGQLDNTFVIFLSDNGAEGALLEAFPKFGPNLQSFLDQHYDNSLDNIGNANSYVWYG
PRWAQAATAPSRLFKAFTTQGGIRVPALLRYPELQRQQGQIDHSFATVMDITPTILDLAG
VRHPGSRWRGREVAPLRGKSWLGWLSGETGAPHDEHHVTGWELFGMRAVRKGSWKAVYIP
APLGPESWQLYDLSKDPGEIHDLAKHEPAKLAALIEHWHEYAEETGVVLGRSPFQPD