Protein Info for OH686_14140 in Pseudomonas sp. S08-1

Annotation: gamma-glutamyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 557 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR00066: gamma-glutamyltransferase" amino acids 30 to 548 (519 residues), 371.5 bits, see alignment E=3e-115 PF01019: G_glu_transpept" amino acids 43 to 551 (509 residues), 514.7 bits, see alignment E=1.5e-158

Best Hits

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 73% identity to pap:PSPA7_0455)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (557 amino acids)

>OH686_14140 gamma-glutamyltransferase (Pseudomonas sp. S08-1)
MLAKHLLAGLLLACTLATQAAPAKPQQAAVATAHPAATVAGLETLAQGGNAFDAAVAIAA
ALAVAEPYGSGLGGGGFFLLRQAGDKPTYRFIDARERAPLSAGPDLYRRNGTVQRELSLN
GPLAAAIPGLPAALAHLAERYGKLPLTDSLTPAIRLARDGVGIDRVYRERAEWRLEALRQ
DPTSAAIFLDQERIPPLHGLLRQPALVRTLERLARDGHAGFYGGETARLLLEGVQAGGGV
WSSRDLRDYRVVERQPLRVTLEGGRELISAPPPSAGGVALAQSLLILQQLPWQKAGAVQR
SHYVVETLRRAYRDRGLLGDPDHVRNPLPHLLAPDYLKGLAKGIALQNATPSASLPPSPA
WREGDHTTHFTVLDAEGNAVAATLSINLPFGAAFTVPGTGVLLNNEMDDFAADPSGSNAY
GLSGSQANVIAAGKRPLSSMSPTFIESEQELASFGTPGGSRIPSMVLLATLDYLEGKPVQ
QWPATPRYHHQYLPDVIEYEADAFSPSQLSSLEMRGHRLKRVERSYGNQQVLLWHKDSGR
VEAASDPRGVGRAELWQ