Protein Info for OH686_13995 in Pseudomonas sp. S08-1

Annotation: polyphosphate kinase 1

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 738 transmembrane" amino acids 470 to 486 (17 residues), see Phobius details TIGR03705: polyphosphate kinase 1" amino acids 57 to 730 (674 residues), 950 bits, see alignment E=3.7e-290 PF13089: PP_kinase_N" amino acids 57 to 162 (106 residues), 123.7 bits, see alignment E=9.8e-40 PF02503: PP_kinase" amino acids 173 to 354 (182 residues), 209.3 bits, see alignment E=1e-65 PF17941: PP_kinase_C_1" amino acids 381 to 544 (164 residues), 236.7 bits, see alignment E=2.7e-74 PF13090: PP_kinase_C" amino acids 553 to 724 (172 residues), 230.9 bits, see alignment E=1.6e-72

Best Hits

Swiss-Prot: 86% identical to PPK1_PSEPK: Polyphosphate kinase (ppk) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 86% identity to pba:PSEBR_a5456)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.1

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (738 amino acids)

>OH686_13995 polyphosphate kinase 1 (Pseudomonas sp. S08-1)
MNTEGLSNKQIADAQLEVEPQAPVEVVAEVVAVPAVEPEPAPAPALVIPGLDDSSLYIHR
ELSQLQFNIRVLEQALDESYPLLERLKFLLIFSSNLDEFFEIRVAGLKKQINFAREQAGA
DGLLPSQVLARISEIAHEQVDRQYALLNDVLLPELAKHQVNFIRRRYWTTKLKTWVRRYF
RDEIAPIITPIGLDPTHPFPLLVNKSLNFIVELEGVDAFGRDSGLAIIPAPRLLPRIIRV
PEEVGGAGDNYVFLSSMIHAHADDLFPGMKVKGCYQFRLTRNADLSVDTEDVEDLARALR
GELFSRRYGDAVRLEVAETCPPQLSDYLLKQFNLSESELYRVNGPVNLTRLFSITGLESH
PELQHAPFTPVIPKLLQNAENIFSVVGKQDILLMHPFESFTPVVDLLRQAAKDPHVLAIK
QTLYRSGANSEIVDALVEAARSGKEVTAVIELRARFDEESNLQLASRLQAAGAVVIYGVV
GFKTHAKMMLILRRENGELRRYAHLGTGNYHAGNAKLYTDYSLLTADEGLGEDVSKLFNQ
LIGMGKTLRMKKLLHAPFTLKKTLLDMITREAQFASEGKPAHIMAKVNSLTDPKIIRALY
KASQMGVRIDLVVRGMCCLRPGVPGVSHNIHVRSIVGRFLEHSRIYYFGNGGDEQLFLSS
ADWMERNLDKRVETCFPMEGKKLITRVKKELESYLSDNTQAWVLQPDGRYQRLTPFGNAN
PRNAQAALLDKLTAPQVR