Protein Info for OH686_11550 in Pseudomonas sp. S08-1

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 44 to 63 (20 residues), see Phobius details TIGR00229: PAS domain S-box protein" amino acids 78 to 202 (125 residues), 59.8 bits, see alignment E=2.9e-20 amino acids 205 to 327 (123 residues), 69.7 bits, see alignment E=2.6e-23 PF00989: PAS" amino acids 82 to 195 (114 residues), 34.9 bits, see alignment E=4.7e-12 amino acids 210 to 317 (108 residues), 51.8 bits, see alignment E=2.7e-17 PF08448: PAS_4" amino acids 88 to 200 (113 residues), 26.8 bits, see alignment E=1.8e-09 amino acids 212 to 319 (108 residues), 28.7 bits, see alignment E=4.5e-10 PF13426: PAS_9" amino acids 104 to 196 (93 residues), 21.9 bits, see alignment E=6.2e-08 amino acids 217 to 318 (102 residues), 60.9 bits, see alignment E=4.4e-20 PF08447: PAS_3" amino acids 104 to 192 (89 residues), 81.1 bits, see alignment E=2e-26 amino acids 228 to 313 (86 residues), 47.9 bits, see alignment E=4.6e-16 PF13188: PAS_8" amino acids 209 to 248 (40 residues), 33.5 bits, see alignment 1e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 328 to 483 (156 residues), 106 bits, see alignment E=1.7e-34 PF00990: GGDEF" amino acids 331 to 481 (151 residues), 142.2 bits, see alignment E=4.6e-45 PF00563: EAL" amino acids 502 to 737 (236 residues), 264.8 bits, see alignment E=2.2e-82

Best Hits

KEGG orthology group: None (inferred from 61% identity to pfv:Psefu_0356)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (750 amino acids)

>OH686_11550 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas sp. S08-1)
MKFQRSPLQLSLFYLIVAALWIFCSDALLLWLGLDRDTLNRYQTLKGVCFVLVTGFALYC
ALNQYRLEQRRARSALKHSEERLALALESAQEGLWDWDMQSGQVYYSRQYCDMLGYTLEE
FGATNDSWLSRLHPDDRERAGQRLKTLLAGRSTHDESSFRMRHRDGGYRWLHYRGQMLLD
AQGQPSRFIGTASDVTRRRADEDSLRQAAAVFDSTLEGVLVTDREQRIVHVNPAFSRITG
YAVDEVLGKLPSLLKSGRHDEAFYQSLWHALNSRGAWSGEVWNRRKDGEVFPMWQCIRSI
RDESGTLTHYVAVFSDVSAIKHSQHELDYLAHHDALTGLPNRLLFGERIEQAIQRGGALL
LLDLDHFKIVNESLGHNTGDQLLKLIGERLSEAIGANVTLARLGGDEFGLLSCDCSEADQ
ASQLALELLDSLAQPFAINGETLFIGASIGISLFPDDGDSVEQLMRNADSALARAKSSGR
QTFSFYSQDMTALAQQRVRLEAELRQALEQDELRVHYQPIHRLDDRRMIGAEALVRWQHP
ERGLVPPGEFIPVAEDSGLIGAIDAWVLEQACTQMRRWQALGLQLEFIAVNVSSRLFSRG
ELDLRVERVLRETGLDPAQLELEVTESAVMDDPQRAEELLTQLHRLGVRLAIDDFGTGYS
SLARLKSLPVHKLKLDQSFVRGLPQDGKDAAIAHAVITLGQSLGLHVLAEGIETEEQATY
LREIGCPFGQGYWFGRPQPAERLLAVQADN