Protein Info for OH686_11330 in Pseudomonas sp. S08-1

Annotation: Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF01266: DAO" amino acids 4 to 35 (32 residues), 29.1 bits, see alignment (E = 2.2e-10) PF00890: FAD_binding_2" amino acids 4 to 38 (35 residues), 23.9 bits, see alignment 6.9e-09 PF01494: FAD_binding_3" amino acids 4 to 345 (342 residues), 81.8 bits, see alignment E=1.8e-26 TIGR01988: ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family" amino acids 4 to 387 (384 residues), 399.9 bits, see alignment E=5.4e-124 PF05834: Lycopene_cycl" amino acids 4 to 34 (31 residues), 23.8 bits, see alignment (E = 7e-09) PF13450: NAD_binding_8" amino acids 7 to 35 (29 residues), 25.6 bits, see alignment (E = 3.7e-09)

Best Hits

KEGG orthology group: None (inferred from 78% identity to pmy:Pmen_3096)

MetaCyc: 44% identical to 5-demethoxyubiquinol-8 5-hydroxylase [multifunctional] (Neisseria meningitidis)
OCTAPRENYL-METHYL-METHOXY-BENZOQ-OH-RXN [EC: 1.14.99.60]; 1.14.13.M56 [EC: 1.14.99.60, 1.14.13.M56]; 2-OCTAPRENYLPHENOL-HYDROX-RXN [EC: 1.14.99.60, 1.14.13.M56, 1.14.13.240]

Predicted SEED Role

"2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase (EC 1.14.13.-)" in subsystem Ubiquinone Biosynthesis (EC 1.14.13.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.14.13.-

Use Curated BLAST to search for 1.14.13.- or 1.14.13.240 or 1.14.13.M56 or 1.14.99.60

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>OH686_11330 Ubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (Pseudomonas sp. S08-1)
MIHDVVIIGAGPAGLCLARALSGHGLSVVLLERQAEQELAEPAFDGREIALTHASQALLE
KLGLWQRLPAEEVAVLRDAQVLNGPSLFALKIRAEQAGAERLGHLVANRAIRRAAFEAVA
ECADVRLRCGVQVGAIRHRERVVELELANDEVLHTGLLVAADSRFSEIRRQLGIGARLKD
FGKSMLVCRMDHQKPHRQTAWEWFGYGQTLALLPLNGNRSSVVLTLPPREIERLRGLHEE
AFAREMERRFDRRLGAMQLASERHAYPLIGAYADKLVGPRCALIGDAAVGMHPVTAHGFN
FGLGSVQCLTEAVLAAQSRGENIGAPALLSRYERQHRLATWPLYQATNLLVDVYTNDRLP
VRLLRGAGLRVAQGLLPLKKGIARHLTALN