Protein Info for OH686_10405 in Pseudomonas sp. S08-1

Annotation: malate synthase G

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01345: malate synthase G
  • amino acids 4 to 722 (719 residues), 1213.8 bits, E=0
• PF20656: MS_N
  • amino acids 16 to 70 (55 residues), 48.2 bits, 1.2e-16
• PF20658: MSG_insertion
  • amino acids 160 to 235 (76 residues), 108.3 bits, E=3.4e-35
• PF01274: MS_TIM-barrel
  • amino acids 337 to 576 (240 residues), 225.1 bits, E=1.5e-70
• PF20659: MS_C
  • amino acids 591 to 673 (83 residues), 83.1 bits, E=4.3e-27

Best Hits

Swiss-Prot: 89% identical to MASZ_PSEA8: Malate synthase G (glcB) from Pseudomonas aeruginosa (strain LESB58)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 91% identity to pmy:Pmen_4096)

MetaCyc: 68% identical to malate synthase G (Mycobacterium tuberculosis H37Rv)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase G (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glyoxylate cycle (5/6 steps found)
• glycolate and glyoxylate degradation II (1/2 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glycol metabolism and degradation (4/7 steps found)
• L-arabinose degradation IV (2/8 steps found)
• D-xylose degradation IV (1/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (1/14 steps found)
• superpathway of pentose and pentitol degradation (8/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Protein Sequence (725 amino acids)

>OH686_10405 malate synthase G (Pseudomonas sp. S08-1)
MTERVQVGGLQVAKALYDFINNEAIPGTGIAADKFWAGAEAVINDLAPKNRALLAKRDEF
QAKIDAWHQARKGQAHDAAAYKAFLQEIGYLLPEPADFQATTQNVDEEIARLAGPQLVVP
VMNARFALNASNARWGSLYDALYGTDAISEEGGAEKGKGYNKVRGDKVIAFARAFLDEAA
PLEGASHVDATAYVVKNGQLAVTLKNGSEAGLKNAAQFIAFQGDAAAPIAVLLKHNGLHF
EIQIDASSPIGQTDAAGVKDVLMEAALTTIMDCEDSVAAVDADDKVIVYRNWLGLMKGDL
AEEVSKGGSTFTRTMNPDRVYTKADGSELTLHGRSLLFVRNVGHLMTNPAILDAQGNEIP
EGIQDGLFTSLIAIHNLNGNTSRKNTRTGSVYIVKPKMHGPEEVAFTGEIFSRVEDVLGL
PRNTLKVGIMDEERRTTVNLKACIKAASERVVFINTGFLDRTGDEIHTSMEAGAVVRKAA
MKAEKWIGAYENWNVDIGLATGLQGRAQIGKGMWAMPDLMAGMLEQKIAHPLAGANTAWV
PSPTAATLHTLHYHKVDVFARQAELAKRERASIDDILSIPLAKDTNWSAEEIQNELDNNS
QGILGYVVRWIDQGVGCSKVPDINDVGLMEDRATLRISSQLLANWLRHGIVTEEQIVASL
KRMAPVVDRQNANDPLYRPLAPNFDSNIAFQAALELVVEGTKQPNGYTEPVLHRRRREFK
AANGL