Protein Info for OH686_10100 in Pseudomonas sp. S08-1

Annotation: ATP-dependent protease HslVU, ATPase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 TIGR00390: ATP-dependent protease HslVU, ATPase subunit" amino acids 12 to 455 (444 residues), 702.5 bits, see alignment E=1.1e-215 PF07728: AAA_5" amino acids 60 to 96 (37 residues), 25.9 bits, see alignment 2.7e-09 PF00004: AAA" amino acids 61 to 145 (85 residues), 35.2 bits, see alignment E=4.8e-12 amino acids 243 to 340 (98 residues), 27.8 bits, see alignment E=9e-10 PF07724: AAA_2" amino acids 196 to 337 (142 residues), 94 bits, see alignment E=3.2e-30

Best Hits

Swiss-Prot: 92% identical to HSLU_PSEMY: ATP-dependent protease ATPase subunit HslU (hslU) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K03667, ATP-dependent HslUV protease ATP-binding subunit HslU (inferred from 92% identity to pmk:MDS_0570)

MetaCyc: 72% identical to ATPase component of the HslVU protease (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent hsl protease ATP-binding subunit HslU" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>OH686_10100 ATP-dependent protease HslVU, ATPase subunit (Pseudomonas sp. S08-1)
MSAASGATYMSMTPREIVSELNRHIIGQDDAKRAVAIALRNRWRRMQLPAELRQEVTPKN
ILMIGPTGVGKTEIARRLAKLANAPFLKVEATKFTEVGYVGRDVESIIRDLADAALKLLR
EQEVQKMSHRAEDAAEERILDALLPPARVGFNDDPAPSQDSNTRQLFRKRLREGQLDDKE
IDIEVAEQPAGVEIMTPPGMEEMTSQLQNLFSGLTKGKKKTRKLKVKEAFKLVRDEEAAR
LVNEEDLKARALEAVEQHGIVFIDEIDKVAKRANVGGADVSREGVQRDLLPLIEGCTVNT
KLGMVKTDHILFIASGAFHLAKPSDLVPELQGRLPIRVELKALSPEDFERILTEPHAALT
EQYSELLKTEGLNIEFAADGIKRIAEIAWQVNEKTENIGARRLHTLLERLLEEVSFSAGD
LAGQQNGEPIRIDAAYVNGHLGELAQDEDLSRYIL