Protein Info for OH686_09800 in Pseudomonas sp. S08-1

Annotation: Mechanosensitive channel MscK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1104 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 491 to 507 (17 residues), see Phobius details amino acids 536 to 560 (25 residues), see Phobius details amino acids 575 to 596 (22 residues), see Phobius details amino acids 614 to 631 (18 residues), see Phobius details amino acids 643 to 665 (23 residues), see Phobius details amino acids 677 to 700 (24 residues), see Phobius details amino acids 712 to 733 (22 residues), see Phobius details amino acids 781 to 804 (24 residues), see Phobius details amino acids 824 to 845 (22 residues), see Phobius details amino acids 866 to 888 (23 residues), see Phobius details amino acids 899 to 926 (28 residues), see Phobius details PF12795: MscS_porin" amino acids 37 to 271 (235 residues), 190.7 bits, see alignment E=8.6e-60 PF12794: MscS_TM" amino acids 491 to 811 (321 residues), 338.2 bits, see alignment E=1.1e-104 PF21088: MS_channel_1st" amino acids 872 to 913 (42 residues), 35.8 bits, see alignment (E = 1.6e-12) PF00924: MS_channel_2nd" amino acids 915 to 980 (66 residues), 80.4 bits, see alignment 2.2e-26 PF21082: MS_channel_3rd" amino acids 988 to 1071 (84 residues), 57.1 bits, see alignment 5.1e-19

Best Hits

KEGG orthology group: K05802, potassium efflux system protein KefA (inferred from 67% identity to pfv:Psefu_0481)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1104 amino acids)

>OH686_09800 Mechanosensitive channel MscK (Pseudomonas sp. S08-1)
MSLLRPLFATLILGLCLAAHPVAAAEPPAAKAVQDSLAALADRKLAEPEQSEIKAQLEQT
LSFLAARDESQQKLDALKVQLRDAPRLTSEAQRELARLKASQPVNVATRYARASVEQLEQ
QLSERSVKLSGWQQDLAEASSLLITAQTRPERAQAEISANQTRAMQINNLLKVGKEAGKS
LTSERRGELNAELAALDAQTALRRQELAGNSLLQDLGSARHELLQERIRRLDQESLDLQA
LISDKRRVSSEKTVAELSREAEKATPDSLLAQESATNLKISDYLLRATERLNQLTRMNLE
TKQQLDSVSQAAQALDEQVSVLKGSLLLAKILYQQKQALPKPQLDRNLADEIADIRLYQF
ELNQQRDKLGDLNAYVDGLLASQPSEQVTPELRKTLLDLATTRNDLLQHMNRELNTLLNE
SITLQLNQKQLQEMIGTLRATLDEQMFWIPSNKPLDLAWLQQMPHLLERQLKTMPWGAGV
RELGAGLVDRPWLFIPLLLLIGGLLWRRSWLYAKLQALHEDIGHYKRDSQMHTPMAIFLN
VLLALPVTLFLTLCGVALLIDARGQNATLGSALIDMAQAWLVFYTLYRILSPGGVAELHF
RWPRPQVALLSKQVRRLGLVVMALVAVVTVAEHQPQALAEDVVGILVVLTGYALMTLILS
KLLLAEPMREQTSPFRLLLGIGVSVLPLAFIVAVGFGYYYTSLKLTDRLINTLYLLILAL
LIEAAFVRGLAVAARRLAYARVLAKRQAQTKENEDGDEVVVEEPAMDIDQINQQSLRLIR
LALLGGFLACMYLVWADLISVFAYLDQINLYQYSAGTGATATQIPISLLDVLGALIIAGI
TAALARNLPGLLEVLVLSKLRLAQGSAYATTTLLSYAIAGIGFVTALSTLGVSWDKLQWL
VAALSVGIGFGMQAIFANFISGLILLFERPIRIGDLVTIGTVTGTVNRIRIRATHITDSD
RKEVIVPNQILLTSQLINWTLTDTVTRVVLVYTVNRGANLELVRKLLLQAAQENSRVLRD
PAPNVQLKTYGASTLEHELKVYVRELGDRGLATDELNRRIDQLFQDNGINIASTPKMDVV
FSRRSAVHPEPADAEEAAPQQPPR