Protein Info for OH686_09235 in Pseudomonas sp. S08-1

Annotation: apolipoprotein N-acyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 512 transmembrane" amino acids 15 to 46 (32 residues), see Phobius details amino acids 58 to 75 (18 residues), see Phobius details amino acids 87 to 110 (24 residues), see Phobius details amino acids 121 to 142 (22 residues), see Phobius details amino acids 161 to 183 (23 residues), see Phobius details amino acids 195 to 213 (19 residues), see Phobius details amino acids 478 to 498 (21 residues), see Phobius details PF20154: LNT_N" amino acids 19 to 181 (163 residues), 125.4 bits, see alignment E=2.5e-40 TIGR00546: apolipoprotein N-acyltransferase" amino acids 61 to 452 (392 residues), 322.4 bits, see alignment E=2.4e-100 PF00795: CN_hydrolase" amino acids 226 to 470 (245 residues), 111.6 bits, see alignment E=4.4e-36

Best Hits

Swiss-Prot: 77% identical to LNT_PSESM: Apolipoprotein N-acyltransferase (lnt) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03820, apolipoprotein N-acyltransferase [EC: 2.3.1.-] (inferred from 78% identity to psb:Psyr_4349)

Predicted SEED Role

"Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE" in subsystem Phosphate metabolism or Copper homeostasis: copper tolerance (EC 2.3.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (512 amino acids)

>OH686_09235 apolipoprotein N-acyltransferase (Pseudomonas sp. S08-1)
MRWITQPGWPGNLLAIAGGACTTLALAPFDIWPLALLAIAIFYLGLRELNPRQALLRGWC
YGLGLFLAGTSWIYVSIHDYGAASPALAGFLTFGFCAGVALFFALPAWLWARWLRRSDAP
LADALAFAALWLAQEAFRGWFLTGFPWLFSGYSQLHGPLAGLAPVGGMWLLSLTLALSAA
LLVNLPRLLGDKPRLAAGLALLLAPWITGLALQNHAWTASKGEPLKVAAIQGNVQQNMKW
DPAQLDAQLALYRDMTLASRPADLIVWPETAVPVLKEFAEGYLKVMGRVAVDRDAALITG
VPVRQSNEHGEKRYYNAITVTGWGEGTYLKQKLVPFGEYVPLQEVLRGLIAFFDLPMSDF
ARGPSDQGPLQAKGLKIAPFICYEVVYPEFAAGLAAQSDLLLTVSNDTWFGTSIGPLQHL
QMAQMRALEAGRWMVRATNNGVTALIDPQGRITAQIPQFQQAVLYGEVQPMQGLTPYLIW
RAWPLAVLCSLLLGWAFVSRRRASRGIAMQTA