Protein Info for OH686_07825 in Pseudomonas sp. S08-1

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 TIGR00422: valine--tRNA ligase" amino acids 1 to 923 (923 residues), 1206.8 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 14 to 621 (608 residues), 521.9 bits, see alignment E=2.8e-160 PF09334: tRNA-synt_1g" amino acids 38 to 89 (52 residues), 21 bits, see alignment 3.3e-08 PF13603: tRNA-synt_1_2" amino acids 243 to 303 (61 residues), 26.7 bits, see alignment 9.2e-10 PF08264: Anticodon_1" amino acids 664 to 817 (154 residues), 142.6 bits, see alignment E=2.2e-45 PF10458: Val_tRNA-synt_C" amino acids 878 to 940 (63 residues), 65.6 bits, see alignment 1e-21

Best Hits

Swiss-Prot: 65% identical to SYV_ALCBS: Valine--tRNA ligase (valS) from Alcanivorax borkumensis (strain ATCC 700651 / DSM 11573 / NCIMB 13689 / SK2)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 65% identity to abo:ABO_0492)

MetaCyc: 63% identical to valine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Valine--tRNA ligase. [EC: 6.1.1.9]; 6.1.1.9 [EC: 6.1.1.9]; 3.1.1.- [EC: 6.1.1.9]; RXN-23924 [EC: 6.1.1.9]

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (943 amino acids)

>OH686_07825 valine--tRNA ligase (Pseudomonas sp. S08-1)
MDKTYQPHAIETSWYQTWEKNNYFAPQGSGEPYTIMIPPPNVTGSLHMGHGFNNSIMDCL
IRFRRMQGRNTLWQPGTDHAGIATQMVVERQLAADGVGRHDLGREKFLEKVWEWKEQSGG
TITRQIRRLGSSVDWSRERFTMDEGLSEAVKEAFVRLHQDGLIYRGKRLVNWDTKFHTAI
SDLEVENHDEKGHLWNLRYPLADGNKTADGKDYLIVATTRPETMLGDSAVAVHPEDERYK
ALIGTFVELPLVGRRIPIIGDDYCDPEFGTGCVKITPAHDFNDYEVGKRHNLPLLNIFDK
NAAVLAKAQVFNIDGSVNTEIDGSLPAEYAGLERFVARKQIVAAFEAAGLLQSIDDHALK
VPKGDRSGTVIEPWLTDQWYVSTKPLAEKAIAVVESGEIQFVPKQYENMYFSWMRDIQDW
CISRQLWWGHRIPAWYDDAGNVYVGRDEAEVRAKHNLGDANLRQDEDVLDTWFSSGLWTF
STLGWPQQTDFLKTFHPTDVLVTGFDIIFFWVARMIMLSTHLTGQIPFKTVYVHGLVRDG
QGQKMSKSKGNVLDPLDIVDGIGLEELLEKRTSGMMQPKLAEKIAKQTRAEFPEGIAAYG
TDALRFTNLALASTGRDIKFDMGRVEGYRNFCNKLWNAANFVIENTDNQDTGINGEPVDL
SPVDRWIISALQRCEQDVTRHLDAFRFDMAAQALYEFIWDEYCAWYLELVKPVLWDENAP
VERQRGTRRTLVRVLEVILRLAHPFMPFITEEIWQRIKGQAGITGETIMLQAWPVANESR
IDAAAEGDIEWVKQLMLGVRQIRGEMKISMAKRIDIIVQNANGDDLRRLADFEPLLSKLA
KLESVRVLAAGEEAPMSATALVGEMQVLVPMAGLIDKDAELARLDKEIQRLQGEVQRVGG
KLSNEGFVAKAPAEVLDKERAKLAEAEQALAKLVGQREKIANL