Protein Info for OH686_07610 in Pseudomonas sp. S08-1

Annotation: tRNA-dihydrouridine synthase DusB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 332 TIGR00737: putative TIM-barrel protein, nifR3 family" amino acids 6 to 321 (316 residues), 422.5 bits, see alignment E=5.3e-131 PF01207: Dus" amino acids 16 to 320 (305 residues), 365.2 bits, see alignment E=1.3e-113

Best Hits

Swiss-Prot: 83% identical to DUSB_PSEAE: tRNA-dihydrouridine synthase B (dusB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K05540, tRNA-dihydrouridine synthase B [EC: 1.-.-.-] (inferred from 87% identity to pmy:Pmen_0710)

MetaCyc: 61% identical to tRNA-dihydrouridine synthase B (Escherichia coli K-12 substr. MG1655)
1.1.1.-

Predicted SEED Role

"tRNA dihydrouridine synthase B (EC 1.-.-.-)" (EC 1.-.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (332 amino acids)

>OH686_07610 tRNA-dihydrouridine synthase DusB (Pseudomonas sp. S08-1)
MSVVRIGPYTLPNQLILAPMAGVTDQPFRQLCRRLGAGLVVSEMVTSDVRLWNSRKSSLR
MIHTGDPEPRSVQIAGGDPEMLAEAARRNVEMGAQIIDINMGCPAKKVCNKAAGSALMKD
EELVTAILNAVVSAVHVPVTLKIRTGWDRANKNGITVAKIAEQAGIAALAVHGRTRADLY
TGEAEYDTIAAIKQAISIPVFANGDIDSPHKAKAVLDATGADALLIGRAAQGRPWIFREI
AHYLATGKELPAPTLYEVERILLEHLAALHAFYGDLMGVRIARKHVGWYLATLPGAREFR
AEFNRLESTDAQYTAARQFFAEQNNKGEGVAA