Protein Info for OH686_05955 in Pseudomonas sp. S08-1

Annotation: (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 292 PF12146: Hydrolase_4" amino acids 27 to 256 (230 residues), 30 bits, see alignment E=5e-11 PF00561: Abhydrolase_1" amino acids 29 to 127 (99 residues), 32.4 bits, see alignment E=1.2e-11 PF12697: Abhydrolase_6" amino acids 30 to 257 (228 residues), 37 bits, see alignment E=8.8e-13

Best Hits

Swiss-Prot: 60% identical to PHAG_PSEAE: (R)-3-hydroxydecanoyl-ACP:CoA transacylase (phaG) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 59% identity to pfs:PFLU1434)

MetaCyc: 58% identical to 3-hydroxyacyl-CoA-acyl carrier protein transferase (Pseudomonas putida KT2440)
2.4.1.-

Predicted SEED Role

"(R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase) (EC 2.4.1.-)" in subsystem Rhamnolipids in Pseudomonas (EC 2.4.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.-

Use Curated BLAST to search for 2.4.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (292 amino acids)

>OH686_05955 (R)-3-hydroxydecanoyl-ACP:CoA transacylase PhaG (3-hydroxyacyl-CoA-acyl carrier protein transferase)  (Pseudomonas sp. S08-1)
MKAETAVLDIQGQYRVYTEFYRADGAEQTIILVNGSLSTTASFAQTVRYLHPHFNVVLFD
LPYAGHSREYNSHDQFLTREDEADILLALVEHFACDTVLSFSWGGIATLQALARKPPRIE
RAVINSFSPVVNSAMLGYLRAGLAVMRDCDRESIGMVLNGTIGKHLPSLFKRYNHRHVTG
LERYEYEQMSFHVETVLNNGGRSCVSFAGQIDIPLLFVNGEWDEYTTPEDARLFTQYVRH
GEFRTIPQAGHFLDMEHKAAWMQTRDALLAFLLPPTTSRARSQTAEKHALAV