Protein Info for OH686_04830 in Pseudomonas sp. S08-1

Annotation: ATP-dependent helicase DinG/Rad3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 715 transmembrane" amino acids 473 to 493 (21 residues), see Phobius details PF00270: DEAD" amino acids 58 to 117 (60 residues), 25.5 bits, see alignment 9.8e-10 PF13307: Helicase_C_2" amino acids 553 to 709 (157 residues), 141.7 bits, see alignment E=2.5e-45

Best Hits

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 89% identity to pmy:Pmen_1427)

Predicted SEED Role

"ATP-dependent helicase DinG/Rad3" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (715 amino acids)

>OH686_04830 ATP-dependent helicase DinG/Rad3 (Pseudomonas sp. S08-1)
MLSSELKSQIQGAYSRFLEAKGLKARYGQRLMIAEVAKVLGAIKHNEEGQRDGEPAVVAV
EAGTGTGKTVAYSLAAIPTAKAAGKRLVIATATVALQEQIVHKDLPDLMRNAGLQFSFAL
AKGRGRYLCLSKLDMLLQEGQAQSATAQMFEEEGFRIDVDENSQKLYSKMIEKLAGNRWD
GDRDSWPEELEDQVWGTLTTDHSQCTNRHCPNFQQCAFYKAREGMTKVDVIVTNHDMVLA
DLALGGGAVLPDPRETLYVFDEGHHLPDKAIGHFAHFTRLRSTADWLGQVEKNLTKLLAQ
HPLPGDLGRLIEQVPEQARELRTQQQFMFSACEQLADFRAGEDMEGRERPRHRFEGGLVP
EHLRELGIELKKGFAKLTDVFTRLTELLKQAMDDEGTSIGLASHQAEEWYPLFGSLLARA
QGNWELWTAFTVEDPEDSPPMARWLTLADSGALFDIEVNASPILAAETLRRNLWNVAYGA
LVTSATLTALGTFDRFRMRSGLPKAAVTAVVPSPFHHADAGVLRVPDLKADPRDAAAHTA
AIIRDLPALVKGARGTLVLYSSRKQMQDVFDGLDRDWRKRVLIQGNLSKQETLNKHKARV
DDGEESVLFGLASFAEGVDLPGAYCEHVVIAKIPFAVPDDPVEAALAEWIEARGGNPFME
IAVPDASLRLIQACGRLLRTEADRGTITLLDRRVVTQRYGKAILNALPPFRREIA