Protein Info for OH686_04555 in Pseudomonas sp. S08-1

Annotation: glutamate--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 86% identical to SYE_PSEMY: Glutamate--tRNA ligase (gltX) from Pseudomonas mendocina (strain ymp)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 86% identity to pmy:Pmen_2733)

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17) @ Glutamyl-tRNA(Gln) synthetase (EC 6.1.1.24)" (EC 6.1.1.17, EC 6.1.1.24)

MetaCyc Pathways

adenosylcobalamin biosynthesis II (aerobic) (31/33 steps found)
tRNA charging (20/21 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (28/36 steps found)
superpathway of heme b biosynthesis from glutamate (9/10 steps found)
tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17 or 6.1.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (494 amino acids)

>OH686_04555 glutamate--tRNA ligase (Pseudomonas sp. S08-1)
MTKVRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGEFILRIEDTDQVRSTRESEQQIFD
ALRWLGIEWNEGPDVGGPHGPYRQSERGHIYKKYSDELVSKGHAFPCFCSAERLDQVRAE
QMAKKETPRYDGHCMHLAPADAQQRIAAGESHVVRMKVPSEGVCVVPDMLRGDVEIPWDR
MDMQVLMKADGLPTYFLANVVDDHLMEITHVLRGEEWLPSAPKLIKLYEYFGWEQPKLCY
MPLLRNPDKSKLSKRKNPTCITFYERMGFMPEALLNYLGRMGWSMPDEREKFSLAEMIEH
FDLSRISLGGPIFDIEKLSWLNGQWLRELPVEQFAQRVRDWAFNSQYLMQIAPHVQQRVE
TFSDIAPLSSFFFSGGVQLDPALLAHKKLSPDQVRQSLQLLLWELEALRQWDKEKITACI
QFVCEGLGLKLRDLMPLIFPAITGKASSVSVLDAMEILGADLSRFRLRQAIELLGGVSNG
ETKEWKKLLEGFRG