Protein Info for OH686_03930 in Pseudomonas sp. S08-1

Annotation: argininosuccinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00032: argininosuccinate synthase
  • amino acids 6 to 398 (393 residues), 469.2 bits, E=6.8e-145
• PF00764: Arginosuc_synth
  • amino acids 6 to 167 (162 residues), 220.8 bits, E=1.1e-69
• PF20979: Arginosuc_syn_C
  • amino acids 178 to 396 (219 residues), 268.6 bits, E=4.1e-84

Best Hits

Swiss-Prot: 97% identical to ASSY_PSEU5: Argininosuccinate synthase (argG) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01940, argininosuccinate synthase [EC: 6.3.4.5] (inferred from 96% identity to pfo:Pfl01_4517)

Predicted SEED Role

"Argininosuccinate synthase (EC 6.3.4.5)" in subsystem Arginine Biosynthesis extended (EC 6.3.4.5)

MetaCyc Pathways

• superpathway of arginine and polyamine biosynthesis (16/17 steps found)
• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
• superpathway of L-citrulline metabolism (9/12 steps found)
• nitric oxide biosynthesis II (mammals) (2/3 steps found)
• urea cycle (3/5 steps found)
• canavanine biosynthesis (2/4 steps found)
• L-arginine biosynthesis IV (archaea) (4/9 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.5

Protein Sequence (405 amino acids)

>OH686_03930 argininosuccinate synthase (Pseudomonas sp. S08-1)
MADVKKVVLAYSGGLDTSVILKWLQDTYNCEVVTFTADLGQGEEVEPARAKAQAMGVKEI
YIDDLREEFVRDFVYPMFRANTIYEGEYLLGTSIARPLIAKRLIEIANETGADAISHGAT
GKGNDQVRFELGAYALKPGVKVIAPWREWDLLSREKLMDYAEKHAIPIERHGKKKSPYSM
DANLLHISYEGGVLEDTWTEHEEDMWKWTKSPEAAPDTPTYIELTYRKGDIVAIDGKDMT
PAAVLAELNRIGGENGIGRLDIVENRYVGMKSRGCYETPGGTIMLRAHRAIESITLDREV
AHLKDELMPKYASLIYTGYWWSPERLMLQQMIDASQTNVNGVVRLKLYKGNVIVVGRKSD
DSLFDANIATFEEDGGAYNQADAAGFIKLNALRMRIAAGKGRKLF