Protein Info for OH686_01305 in Pseudomonas sp. S08-1

Annotation: glycogen debranching enzyme GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 708 TIGR02100: glycogen debranching enzyme GlgX" amino acids 11 to 702 (692 residues), 1104.3 bits, see alignment E=0 PF02922: CBM_48" amino acids 15 to 103 (89 residues), 71 bits, see alignment E=8.8e-24 PF00128: Alpha-amylase" amino acids 197 to 283 (87 residues), 33.4 bits, see alignment E=4e-12

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 86% identity to pmk:MDS_2452)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (708 amino acids)

>OH686_01305 glycogen debranching enzyme GlgX (Pseudomonas sp. S08-1)
MGKQRSRIAEGHPFPLGATWDGLGVNFALFSAHATKVELCLFDDQGKTEIERIELPEYTD
EIWHGYLPDAHPGQVYGYRVYGPYEPDAGHRFNPNKLLIDPYAKQLVGKLQWSEALFGYT
IGHPDADLSFDERDSAAFVPKSKVMDPAFTWGEQAPIRVPWDSTILYETHLRGISMLHPA
VPENLRGTCAGLMNPELLKHIRSLGVSSIELLPVHAFVNDQHLLEKGMNNYWGYNSIGFF
APHPAYLASGKVSEFKEMVAHLHDAGLELILDVVYNHTAEGNERGPTLCMRGIDNASYYR
LMPDDRRYYINDSGTGNTLDLSHPCVLQMVTDSLRYWATEMRVDGFRFDLATILGRYADG
FDERHSFLVACRQDPVLSKVKLIAEPWDCGPGGYQVGGFPPGWAEWNDKFRDNARAFWKG
DEGQLAELASRLTASGDLFNQRGRRPFASVNFITAHDGFTLRDVVSYDHKHNEANDEDNR
DGTDHNISWNHGCEGPSDDEQIRELRLRQMRNLLATLLFAQGTPMLLAGDEFGRTQQGNN
NAYCQDNELSWVDWNIDEEGQGLIAFTRRLIALRRAYPILRRGRFFVGHYNEELGVKDVT
WLAPGGEEMTEEHWHDGEARCMGMLLDGRAQPTGIRRSGADATLLLILNAYHEAVDFLLP
EVAEGSAWIGLLDSNQPQQRSTEQFAFGSQFQVAPRSLLLFKLQKESD