Protein Info for OH686_00995 in Pseudomonas sp. S08-1

Annotation: diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1194 PF00072: Response_reg" amino acids 9 to 121 (113 residues), 94.8 bits, see alignment E=1.8e-30 amino acids 1061 to 1170 (110 residues), 52 bits, see alignment E=3.7e-17 PF13185: GAF_2" amino acids 203 to 339 (137 residues), 36.9 bits, see alignment E=2.1e-12 PF01590: GAF" amino acids 274 to 339 (66 residues), 24.4 bits, see alignment 1.9e-08 TIGR00229: PAS domain S-box protein" amino acids 361 to 485 (125 residues), 25.5 bits, see alignment E=1.2e-09 amino acids 495 to 615 (121 residues), 78.5 bits, see alignment E=4.9e-26 PF08448: PAS_4" amino acids 370 to 485 (116 residues), 39.6 bits, see alignment E=2.8e-13 amino acids 500 to 610 (111 residues), 44.4 bits, see alignment E=8.9e-15 PF00989: PAS" amino acids 495 to 605 (111 residues), 60.8 bits, see alignment E=6.3e-20 PF13188: PAS_8" amino acids 495 to 546 (52 residues), 28 bits, see alignment (E = 7.9e-10) PF13426: PAS_9" amino acids 503 to 607 (105 residues), 78.8 bits, see alignment E=1.7e-25 PF08447: PAS_3" amino acids 516 to 601 (86 residues), 37.1 bits, see alignment 1.6e-12 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 615 to 778 (164 residues), 155.5 bits, see alignment E=1e-49 PF00990: GGDEF" amino acids 619 to 776 (158 residues), 164.3 bits, see alignment E=1e-51 PF00563: EAL" amino acids 796 to 1030 (235 residues), 248.7 bits, see alignment E=2.6e-77

Best Hits

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s)" in subsystem Bacterial hemoglobins

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1194 amino acids)

>OH686_00995 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) (Pseudomonas sp. S08-1)
MTMYADAEILVVEDSHTQATELQYMLEAVGCKVRVASNGREALEMVAVRKPTIVISDIVM
PEMDGYELCRQLKSSALTQGIPIILVTALADARDVVHGLASGADNFIVKPFDEKYLMSRI
RYFLVNLELRTHERVQMGVEVVLEGERHFITASRQQILDLLISTYEQGVRLNNELQAKHD
ELARTNSLLNCLFHFTSGLTDAKSERNVIDAALRRILEFPDAVGAWLLLADANEPHEPAH
VAGACARGQNYSKHDLDSCARNCPCRHAWVSDKLSRPTNISDCPALLDKPGDSIHASIPL
QMGGEIIGMLNVVQRDAQPWSEDSLSALASIGRQLAMALGRVRLFESMEQLVEQRTEALT
KSEELLRKVLDSLPVGVFVTDQQGRLIMSNPESSLIWGGAHPSGADGYGHYRGWWAETGE
RITESGWSMMRAVQQGRVSRNEVVDIETFKGERKTILNSAVPFRDASGVIQGAIVVIQDI
TEQRRRDLEIRIRTRAVEASVNAVIITDAEHPDQPIVYANPAFERITGYSVDEVLGRNCR
FLQGKDHDQAELDSIRRALAAKEEGAALLRNFRKDGSEFWNDLRVAPVVNDRGKVSHFVG
ILHDVTESKRYQEELEHQANHDSLTGLPNRNLLNDRIGQAIAFASRGSGQFTLAFMDIDQ
FKVVNDSLGHSSGDQLLIEIAARLRASVRDIDTVARLGGDEFIILLADTDQLSEQLALLE
QIKDSIASPMMLDGQEVAVSCSIGFCCFPSDGRDAGTLLRNADTAMYQAKHQGRNRICAF
MPAMNEQVQARLAMEQQTRHALQNQEFIVFYQPQLDLQNGRLCGFEALVRWNRGGTMVSP
GEFIPLAEETGWIVDIDFYVFEMVCKQLAIWRESFSQDLSVAVNFSAISFADENFIERIL
SVLRSQNIPSRWIKIELTETILTSDADVVQRKMQHLCEQGVRFSIDDFGTGYSSLGYLKR
FPFSQLKIDRSFITDILTEPDSASLARSMISIGHNLGIKVIAEGVEQSEQMSFLRAAGCD
EMQGYFYSPPLPPLACMQFLQSGAPLSLPANILDGTERYLLIVDGSAESHEALRRVLRLE
SYKLLTASDGAAALNLMALYPVDVVLTDLSLVDMHGVEFLRKIRSIYPDAIRLALSDAAR
VGSILKAVNEGVIYRFITTPWEPEDLRNQLREAFQQRELHRENRRLRQQIGMAR