Protein Info for OH686_00170 in Pseudomonas sp. S08-1

Annotation: Isocitrate lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00463: ICL
  • amino acids 86 to 270 (185 residues), 70.9 bits, E=8.4e-24
  • amino acids 333 to 392 (60 residues), 36 bits, E=3.2e-13
  • amino acids 428 to 530 (103 residues), 49.7 bits, E=2.3e-17
• PF13714: PEP_mutase
  • amino acids 174 to 267 (94 residues), 44 bits, E=2.2e-15

Best Hits

Swiss-Prot: 92% identical to ACEA_PSEAE: Isocitrate lyase (PA2634) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01637, isocitrate lyase [EC: 4.1.3.1] (inferred from 97% identity to pmk:MDS_2362)

Predicted SEED Role

"Isocitrate lyase (EC 4.1.3.1)" in subsystem Serine-glyoxylate cycle (EC 4.1.3.1)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (21/26 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glyoxylate cycle (5/6 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.1

Protein Sequence (531 amino acids)

>OH686_00170 Isocitrate lyase  (Pseudomonas sp. S08-1)
MSAYQNDIKAVAALKEAAGSSWSAINPESVARMRAQNRFKTGLEIAQYTADIMRKDMAEY
DADSSKYTQSLGCWHGFIGQQKLIAIKKHLKTTNKRYLYLSGWMVAALRSDFGPLPDQSM
HEKTAVSDLIEELYTFLRQADSRELDLLFTALDAARAAGDNAKAAEIQNQIDNYETHIVP
IIADIDAGFGNPEATYLLAKRMIEAGACCIQIENQVSDEKQCGHQDGKVTVPHADFLAKI
AAVRYAFLELGIDNGVIVARTDSLGAGLTKQIAVTKEPGDLGDQYNSFLDCEEVAASDLG
NGDVVINRGGKLLRPKRLPSNLFQFRAGTGEDRCVLDCITSLQNGADLLWIETEKPHVGQ
IKGMVDRIREVIPNAKLVYNNSPSFNWTLNFRQQVFDAFAAEGKDVSAYDRAKLMSVEYD
ETELAQVADEKIRTFQRDGSAHAGIFHHLITLPTYHTAALSTDNLAKGYFADQGMLAYVK
GVQRQELRQGIACVKHQNMAGSDIGDNHKEYFAGEAALKASGKDNTMNQFH