Protein Info for NOLOHH_23630 in Escherichia coli ECOR27

Annotation: rhomboid family intramembrane serine protease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 44 to 62 (19 residues), see Phobius details amino acids 69 to 91 (23 residues), see Phobius details amino acids 100 to 121 (22 residues), see Phobius details amino acids 127 to 150 (24 residues), see Phobius details amino acids 161 to 178 (18 residues), see Phobius details amino acids 184 to 202 (19 residues), see Phobius details amino acids 212 to 231 (20 residues), see Phobius details PF01694: Rhomboid" amino acids 58 to 202 (145 residues), 101.6 bits, see alignment E=6.8e-33 PF10282: Lactonase" amino acids 301 to 421 (121 residues), 23.2 bits, see alignment E=5.5e-09 TIGR02276: 40-residue YVTN family beta-propeller repeat" amino acids 361 to 396 (36 residues), 27.9 bits, see alignment 8.8e-11

Best Hits

KEGG orthology group: None (inferred from 97% identity to sbc:SbBS512_E0591)

Predicted SEED Role

"Putative membrane protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (543 amino acids)

>NOLOHH_23630 rhomboid family intramembrane serine protease (Escherichia coli ECOR27)
MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSALIHLGANVASLTLSGE
SWRLLSSVFLHSSVSHLLMNMFALLVVGGVVERILGKWRLLIVWLFSGVFGGLISACYAL
RESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQRRVFPLLGMVALTLLYGARQT
GIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIVAGSLLLTGAIWLAQQQMDESVL
QVRQSLREAFYPQEIEQERRQKKQQLAEERNALRETLSAPVSREQASGDLLAEIADIHDM
AISRDGNTLYAAIENTNSIVVFDLGQKKILHTFTAPIAKEKSVKHCGGCKDQGVRSLALS
PDEKLIYATSFEANALSVINVATGEIIQSITTGAHPDSFILSRDGTKAWVMNRTSNSVSA
IDLVTYQHVADIPLEKYDGTGTSGKPGAWVMALSPDERTLLVPGAGRGNIVRINTITHQK
EDFPAGDARGTISAMRFRPENGEVIFADSQGISRISVGDQQASIMTQWCSRSVYSVEGIS
PDG