Protein Info for NOLOHH_22440 in Escherichia coli ECOR27

Name: pdeI
Annotation: putative cyclic di-GMP phosphodiesterase PdeI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 782 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 286 to 305 (20 residues), see Phobius details PF17153: CHASE9" amino acids 35 to 150 (116 residues), 208.7 bits, see alignment E=1.8e-66 PF00563: EAL" amino acids 532 to 767 (236 residues), 232.7 bits, see alignment E=4.3e-73

Best Hits

Swiss-Prot: 100% identical to PDEI_ECOLI: Probable cyclic di-GMP phosphodiesterase PdeI (pdeI) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eck:EC55989_0878)

Predicted SEED Role

"FIG00638993: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (782 amino acids)

>NOLOHH_22440 putative cyclic di-GMP phosphodiesterase PdeI (Escherichia coli ECOR27)
MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKD
IERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVV
YHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLR
INGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYN
SEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLV
VYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAE
IERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIV
RKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKS
AICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEI
GELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKT
AMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETV
IERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAG
KIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLV
KE