Protein Info for NOLOHH_20995 in Escherichia coli ECOR27

Name: mfd
Annotation: transcription-repair coupling factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1164 PF17757: UvrB_inter" amino acids 145 to 233 (89 residues), 91.1 bits, see alignment 1.6e-29 TIGR00580: transcription-repair coupling factor" amino acids 161 to 1095 (935 residues), 1243.9 bits, see alignment E=0 PF21132: MFD_D3" amino acids 393 to 465 (73 residues), 97.2 bits, see alignment 1.8e-31 PF02559: CarD_TRCF_RID" amino acids 493 to 548 (56 residues), 68 bits, see alignment 2.4e-22 PF04851: ResIII" amino acids 616 to 774 (159 residues), 51.7 bits, see alignment E=4e-17 PF00270: DEAD" amino acids 618 to 780 (163 residues), 99.9 bits, see alignment E=5.6e-32 PF00271: Helicase_C" amino acids 818 to 922 (105 residues), 70.4 bits, see alignment E=6.2e-23 PF03461: TRCF" amino acids 1022 to 1114 (93 residues), 101.8 bits, see alignment E=8.8e-33

Best Hits

Swiss-Prot: 100% identical to MFD_ECOLI: Transcription-repair-coupling factor (mfd) from Escherichia coli (strain K12)

KEGG orthology group: K03723, transcription-repair coupling factor (superfamily II helicase) [EC: 3.6.4.-] (inferred from 65% identity to asu:Asuc_0557)

Predicted SEED Role

"Transcription-repair coupling factor" in subsystem DNA-replication or Transcription factors bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1164 amino acids)

>NOLOHH_20995 transcription-repair coupling factor (Escherichia coli ECOR27)
MPPYVEAYPNENLTTVMPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQRG
VLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHGEYAT
RGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFPTDKAAI
ELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYFPANTLLVN
TGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNWPRVQLKTEHL
PTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESEGRREALGELLAR
IKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLLGERVARRRQDSRRT
INPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEYLMLTYANDAKLYVPVS
SLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAAELLDIYAQRAAKEGFAFK
HDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDRLVCGDVGFGKTEVAMRAAFL
AVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEMISRFRSAKEQTQILAEVAEGKI
DILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKERIKAMRANVDILTLTATPIPRTLN
MAMSGMRDLSIIATPPARRLAVKTFVREYDSLVVREAILREILRGGQVYYLYNDVENIQK
AAERLAELVPEARIAIGHGQMRERELERVMNDFHHQRFNVLVCTTIIETGIDIPTANTII
IERADHFGLAQLHQLRGRVGRSHHQAYAWLLTPHPKAMTTDAQKRLEAIASLEDLGAGFA
LATHDLEIRGAGELLGEEQSGSMETIGFSLYMELLENAVDALKAGREPSLEDLTSQQTEV
ELRMPSLLPDDFIPDVNTRLSFYKRIASAKTENELEEIKVELIDRFGLLPDPARTLLDVA
RLRQQAQKLGIRKLEGNEKGGVIEFAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQD
LSERKTRIEWVRQFMRELEENAIA