Protein Info for NOLOHH_20705 in Escherichia coli ECOR27

Name: ldcA
Annotation: muramoyltetrapeptide carboxypeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 PF02016: Peptidase_S66" amino acids 5 to 126 (122 residues), 71.4 bits, see alignment E=7.4e-24 PF17676: Peptidase_S66C" amino acids 169 to 283 (115 residues), 108.5 bits, see alignment E=3.3e-35

Best Hits

Swiss-Prot: 98% identical to LDCA_ECOL6: Murein tetrapeptide carboxypeptidase (ldcA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K01297, muramoyltetrapeptide carboxypeptidase [EC: 3.4.17.13] (inferred from 100% identity to eoj:ECO26_1705)

MetaCyc: 98% identical to murein tetrapeptide carboxypeptidase (Escherichia coli K-12 substr. MG1655)
Muramoyltetrapeptide carboxypeptidase. [EC: 3.4.17.13]; RXN0-2061 [EC: 3.4.17.13]

Predicted SEED Role

"Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13)" (EC 3.4.17.13)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.17.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (304 amino acids)

>NOLOHH_20705 muramoyltetrapeptide carboxypeptidase (Escherichia coli ECOR27)
MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCERFAGTETERLEDLNSL
ARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLICGHSDFTAIQCGLLAQGN
VITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQGEGPTCQTEGTLWGGNLAML
ISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYHAGILPRQKAIILGSFSGSTPND
YDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVTLPLGAHAILNNTREGTQLTISGHP
VLKM